UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 13 / No. 1 / Published : 2006-11

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Original Article :

The comparison of wet mount and gram stain method for vaginal smear in bacterial vaginosis

Author :

  1. Paulus B Notopoero*1
  2. Prihatini*2
  1. Bagian Patologi Klinik FK UNAIR-RSU dr. Soetomo
  2. Bagian Patologi Klinik FK UNAIR-RSU dr. Soetomo

Abstract :

Bacterial vaginosis (BV) is a clinical condition with changes in the vaginal ecosystem. Under normal conditions, the vaginal ecosystem contains Lactobacilli microflora but in BV condition, it contains mixed microflora ie combination of anaerobic bacteria and Gardnerella sp. There are approximately 300 cases of BV a year in the Dr. Soetomo General Hospital. We can examine vaginal fluid microscopically to diagnose BV with wet mount and Gram stain method. Wet mount method is fast to do that clinicians can establish the diagnosis and therapy earlier. Gram stain method is the gold standard method and more common to do in the laboratory but the staining method can affect the result. This study aims to know the diagnostic value of wet mount method compared with Gram stain method for BV. There were 30 subjects from the Gynaecology Outpatient Clinic in the Dr. Soetomo General Hospital. They presented with from the leucorrhoea and fullfiled the clinical criteria for BV. We took the vaginal fluid and examined them with wet mount and Gram stain microscopy. The percentage of agreement between wet mount and Gram stain method for BV Grade I is 66%, BV grade II is 66%, BV grade III is 84.6%, and BV grade IV is 100%. The sensitivity of wet mount method is 85.71%, the specificity is 88.88%, the positive predictive value and negative predictive value are 94.73% and 72.72%. Based on these data, wet mount method can replace Gram stain method to diagnose BV microscopically in the case that there is not enough time and resource for Gram stain. The wet mount method has good sensitivity, specificity, positive and negative predictive value. Wet mount method has a shortcoming in identifying the microorganism, but this problem can be solved by combining this method with Gram stain method.

Keyword :

Bacterial vaginosis, wet mount method, Gram stain method.,


References :

Scwebke JR,(1996) Validity of the Vaginal Gram Stain for the Diagnosis of Bacterial Vaginosis. Obstet Gynecol; 88 - : -

Hillier SL,(1996) Validity of the Vaginal Gram Stain for the Diagnosis of Bacterial Vaginosis. Obstet Gynecol; 88 - : -

Sobel JD,(1996) Validity of the Vaginal Gram Stain for the Diagnosis of Bacterial Vaginosis. Obstet Gynecol; 88 - : -

McGregor J,(1996) Validity of the Vaginal Gram Stain for the Diagnosis of Bacterial Vaginosis. Obstet Gynecol; 88 - : -

Sweet R,(1996) Validity of the Vaginal Gram Stain for the Diagnosis of Bacterial Vaginosis. Obstet Gynecol; 88 - : -

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Spiegel CA,(1983) Diagnosis of Bacterial Vaginosis by Direct Gram Stain of Vaginal Fluid. J Clin Microbiol.18

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Amsel R,(1983) Diagnosis of Bacterial Vaginosis by Direct Gram Stain of Vaginal Fluid. J Clin Microbiol.18 - : -

Holmes KK,(



Archive Article

Cover Media Content

Volume : 13 / No. : 1 / Pub. : 2006-11
  1. Levels Of B-hcg Among Patients With Hydatiform Mole Before And After Curettage
  2. Colony Count Candida Albicans Of Stool In Autism Spectrum Disorders
  3. The Comparison Of Wet Mount And Gram Stain Method For Vaginal Smear In Bacterial Vaginosis
  4. Microbial Patterns Based On Type Of Spesimens And Its Sensitivity To Antimicrobial Drugs
  5. The Value Of Sdldl Of Youngsters And Its Correlation With Other Lipids
  6. Lipid Profile In Type 2 Diabetic Mellitus Patient’s
  7. Pathogenesis And Diagnostics Factors Of Von Willebrand Disease
  8. Limited Systemic Sclerosis In A Young Boy
  9. The Correlation Between Fecal Occult Blood Test Using Anti-human Hemoglobin And Microscopic Examination
  10. Advantage And Disadvantage Of Quality Assurance Based On Point Of Care Testing/poct

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