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Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 13 / No. 1 / Published : 2006-11

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Original Article :

The correlation between fecal occult blood test using anti-human hemoglobin and microscopic examination

Author :

  1. Liana*1
  2. Prihatini*2
  1. Bagian Patologi Klinik, Fakultas Kedokteran Universitas Airlangga, RSU Dr. Soetomo Surabaya
  2. Bagian Patologi Klinik, Fakultas Kedokteran Universitas Airlangga, RSU Dr. Soetomo Surabaya

Abstract :

Test for occult blood in faeces is an important part of the early detection of colorectal cancer, gastrointestinal bleeding, and anaemia. Colorectal cancer is the second leading cause of cancer deaths in the United States. Immunochemistry method for detection of human haemoglobin in faeces has been developed. The advantages of this method are improving analytical sensitivity and specificity, also avoiding the dietary restrictions requirement, compared with benzidine test, and guaiacum test. A study was performed to correlate the result of fecal occult blood by immunochemistry method using anti-human haemoglobin and microscopic examination of red blood cells in faeces of outpatients in the Clinical Pathology Laboratory, Dr. Soetomo Hospital, Surabaya. Faeces of fifty one patients tested for fecal occult blood were examined by immunochemistry method compared with microscopic examination of red blood cells. Comparison of the two methods was done by statistical analysis, Mc Nemar test. The correlation was measured using ROC curve. The results showed a correlation between immunochemistry method and microscopic examination with average red blood cells (RBC) ≤ 2/hpf, p = 0.008; RBC ≥ 3/hpf, p = 0.289. ROC curve showed r = 0.941. In conclusion, a significant correlation between positive results of immunochemistry method and microscopic examination with average red blood cells ≥ 3/hpf. Further research using larger and more representative samples should be carried out.

Keyword :

Fecal occult blood, anti-human haemoglobin, microscopic examination,


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Archive Article

Cover Media Content

Volume : 13 / No. : 1 / Pub. : 2006-11
  1. Levels of b-hcg among patients with hydatiform mole before and after curettage
  2. Colony count candida albicans of stool in autism spectrum disorders
  3. The comparison of wet mount and gram stain method for vaginal smear in bacterial vaginosis
  4. Microbial patterns based on type of spesimens and its sensitivity to antimicrobial drugs
  5. The value of sdldl of youngsters and its correlation with other lipids
  6. Lipid profile in type 2 diabetic mellitus patient’s
  7. Pathogenesis and diagnostics factors of von willebrand disease
  8. Limited systemic sclerosis in a young boy
  9. The correlation between fecal occult blood test using anti-human hemoglobin and microscopic examination
  10. Advantage and disadvantage of quality assurance based on point of care testing/poct