UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 15 / No. 1 / Published : 2008-11

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Original Article :

The effect of cryopreservation to epidermal growth factor (egf) level in amnion membrane

Author :

  1. Ety Retno S*1
  2. Gunawan Effendi*2
  3. Gatut Suhendro*3
  4. I. Handojo*4
  1. Departemen Patologi Klinik FK-Unair-RSU Dr. Soetomo, Jl. Prof. Dr. Moestopo 6–8 Surabaya
  2. Departemen Ilmu Kesehatan Mata FK-Unair-RSU Dr. Soetomo, Jl. Prof. Dr. Moestopo 6-8 Surabaya
  3. Departemen Ilmu Kesehatan Mata FK-Unair-RSU Dr. Soetomo, Jl. Prof. Dr. Moestopo 6-8 Surabaya
  4. Departemen Patologi Klinik FK-Unair-RSU Dr. Soetomo, Jl. Prof. Dr. Moestopo 6–8 Surabaya

Abstract :

To measure the difference of EGF concentration in between fresh amniotic membranes and with cryopreservation in 16 amniotic membranes. Each amniotic membrane was divided into two parts. The first part was extracted in fresh forms and the second part underwent cryopreservation with glycerol 50% and was stored at –80° C during 1 month before extraction. Both parts of the membrane were extracted using ultrasonic disintegrator and concentration of EGF was measured from the obtained extract using ELISA method. The average concentration of EGF in the fresh amniotic membrane was 122.76 ± 11.59 pg/g while the average concentration of EGF in the amniotic membrane underwent cryopreservation was 99.34 ± 9.49 pg/g. Average degradation of EGF concentration due to cryopreservation is 18.49% ± 10.20%. EGF concentration in fresh amniotic membrane is significantly higher than the EGF concentration in amniotic membrane underwent cryopreservation (p = 0.000). Degradation of EGF concentration due to cryopreservation at 95% confidence interval is 12.33% to 24.66%.

Keyword :

epidermal growth factor - amniotic membrane - cryopreservation - ultrasonic disintegrator - ELISA,


References :

Tseng SGC, Tsubota K,(2002) Amniotic Membrane tranplantation for ocular surface reconstruction New York : Spinger-Verlag





Archive Article

Cover Media Content

Volume : 15 / No. : 1 / Pub. : 2008-11
  1. (anemia And Iron Deficiency Among Female Adolescents From Junior High School (sltp) Negeri I Curug, Tangerang)
  2. Sgot, Sgpt Activities In Medium And Severe Burn Injuries Patients
  3. Molecular Detection Of Mycobacterium Tuberculosis In Sputum With Polymerase Chain Reaction
  4. The Effect Of Cryopreservation To Epidermal Growth Factor (egf) Level In Amnion Membrane
  5. The Problem Of Vancomycin-resistant Enterococci
  6. Diagnosis Of Filariasis Based On Thick Smear

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