UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 21 / No. 2 / Published : 2015-03

Order : 13, and page :174 - 178

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Original Article :

β-thalassemia trait using capillary electrophoresis

Author :

  1. Nuryanti*1
  2. Ratna Akbar Ganie*2
  3. Adi Koesoema Aman*3
  1. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Sumatera Utara/RSUP H. Adam Malik Medan
  2. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Sumatera Utara/RSUP H. Adam Malik Medan
  3. Departemen Patologi Klinik, Fakultas Kedokteran Universitas Sumatera Utara/RSUP H. Adam Malik Medan

Abstract :

Thalassemia is a genetic disorder disease which spread in the different parts of the world, including Indonesia. The incidence of β- thalassemia trait in Indonesia is between 3−8%. The objective of this study is to know the incidence of β-thalassemia trait in students who performed medical check-up, which obtain by using capilary electrophoresis, to describe the characteristics and to obtain the average value, of MCV, MCH and HbA2. This study was an observational study by cross-sectional method, which was carried out at the Department of Clinical Pathology Haji Adam Malik Hospital in Medan, performed on July to September 2012, consisting of 560 subjects. The examination included FBC to get a microcytic hypochromic sample (MCV <80 fl, MCH <27 pg), then a quantification of HbA2 from microcytic hypochromic sample was done using electrophoresis. From the 560 samples 54% were male and other 46% were female. The average age was 19.25±0.25 years, the percentage based on the races tribe, which including Bataknesse 57.78%, Javanesse 15.74%, Acehnesse 10.73, Malay 9.12%, Karonesse 3.93%, Padangnesse 2.32% and Nias 0.35%. The average of the hematological indices, in the 50 subjects were 71.63±7.68 for MCV and 23.27±2.85 for MCH. The quantification of HbA2 in micrositic hypochromic subject showed 10 β-thalassemia trait subject (1.8%) with average of HbA2 (4.34±0.25), MCV (62.66±3.41), and MCH (20.11±2.18) as well as four (4) hemoglobin E subjects (0.7%). Based on this performed research on 560 students with the incidence of β-Thalassemia Trait was 1.8% with the average of HbA2 quantification was 4.34±0.25. Penyakit talasemia merupakan penyakit akibat kelainan genetik yang tersebar di belahan dunia termasuk Indonesia. Angka kejadian β-thalassemia trait di Indonesia berkisar antara 3−8%. Penelitian ini untuk mengetahui cara mendapatkan angka kejadian β-thalassemia trait di mahasiswa yang memeriksakan kesehatan dengan menggunakan elektroforesis mikrokapiler, guna dapat menggambarkan ciri subjek dan mendapatkan nilai rerata MCV, MCH dan HbA2. Penelitian ini merupakan kajian pengamatan dengan metode potong lintang, dilakukan di Departemen Patologi Klinik RSUP Haji Adam Malik Medan, pada bulan Juli−September 2012, dengan jumlah subjek penelitian 560. Pemeriksaan meliputi full Blood Count (FBC) untuk mendapatkan sampel mikrositer hipokrom (MCV <80 fl, MCH <27 pg), kemudian diperiksa banyaknya HbA2 di sampel mikrositer hipokrom dengan menggunakan metode elektroforesis mikrokapiler. Dari 560 sampel, dijumpai laki-laki 54% dan perempuan 46%. Mereka rerata berumur 19,25± 0,25 tahun, dengan persentase berdasarkan persukuannya, yaitu: suku Batak 57,78%, Jawa 15,74%, Aceh 10,73, Melayu 9,12%, Karo 3,93%, Padang 2,32% dan Nias 0,35%. Hasil memeriksa indeks terkait hematologis, di 50 subjek dengan MCV 71,63±7,68 dan MCH 23,27±2,85, hasil memeriksa terkait banyaknya HbA2 dijumpai 10 yang bernilai HbA2 (4,34±0,25 %) merupakan β-thalassemia trait (1,8 %) dengan MCV (62,66±3,41), MCH (20,11±2,18). Didasari telitian di 560 orang mahasiswa yang telah dilakukan, ditemukan angka kejadian β-Thalassemia Trait 1,8% dengan rerata kadar HbA2 4,34±0,25%.

Keyword :

β-Thalassemia trait, HbA2, capilary electrophoresis,


References :

World Health Organization/Thalassemia International Federation,(1994) Prosiding dari: Joint meeting on the prevention and control of haemoglobinopathies 1 : WHO

Ganie RA,(2004) Kajian DNA thalassemia Alpha di Medan 1-4 : USU Press





Archive Article

Cover Media Content

Volume : 21 / No. : 2 / Pub. : 2015-03
  1. Pneumatic Tube On Routine Blood Test And Lactate Dehydrogenase
  2. Detection In Tetrazolium Microplate Assay Culture Methods From Pulmonary Tuberculosis Suspected Sputum
  3. High Molecular Weight Adiponectin And Vasculer Thickness In Diabetes Type 2 Related To Fixed Dose Combination Of Glimepiride And Metformin
  4. Neutrofil/lymphocyte Ratio In Carcinoma Mammae
  5. Platelet Aggregation And Mean Volume With Metabolic Syndrome In Obesity
  6. Diagnosis Of Pulmonary Tuberculosis Based On Frequency Of Sputum Examination
  7. Genetic Variation Of Hepatitis B Virus Polymerase Gene From Chronic Hepatitis B Infected Patient With Telbivudine Therapy
  8. The 38 Kda Adhesin Protein Of Mycobacterium Tuberculosis And Macrophage Of The Lung
  9. Bacterial Pattern And Patient’s Age On Procalcitonin In Community And Hospital Acquired Pneumonia
  10. Aspergillus Glaucus Group And Penicillium Sp In Neurosurgery Operating Theater
  11. Diagnostic Value Of Iga Antivca Epstein-barr Antibody In Nasophryngeal Carcinoma
  12. Blood Glucose Test Between Hexokinase With Glucose Oxidase And Glucose Dehydrogenase Methods In Diabetes Mellitus
  13. β-thalassemia Trait Using Capillary Electrophoresis
  14. Lipoprotein(a) In Acute Coronary Syndrome
  15. D-dimer Plasma Levels In Ishemic Stroke
  16. Adrenomedullin’s In Breast Cancer With Metastatic State
  17. Storage Temperature For 24 Hours Of Uric Acid In Urine
  18. Ebola Virus Disease
  19. Congenital Malaria

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