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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 44 / No. 1 / Published : 2011-03

Order : 10, and page :49 - 53

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Original Article :

tnf- α expression on rats after inoculated with candida albicans and fed with neem extract (azadirachta indica)

Author :

  1. I Dewa Ayu Ratna Dewanti*1
  1. Bagian Biomedik, Fakultas Kedokteran Gigi Universitas Jember

Abstract :

     Background: Neem is a known traditional medicine from trees which function as immunomodulator. Candidiasis found in mouth is 80% caused by Candida albicans (C. albicans). Immunity is important to limit C. albicans since medicine price is relatively traditional medicine may become a good choice. In the other side the medicine price may not be reached by the citizen, cause citizen choose the traditional medicine. Purpose: This article is aimed to explain of  TNF- α expression on rats after  inoculated by C. albicans and fed with neem extract (Azadirachta Indica). Methods: There were 5 groups, the first group which was called as control group (KO) hadn’t been fed aqueous extract  from neem leaves and was not inoculated by C. albicans, the other group (treatment) was classified into 4 groups. The first group was inoculated by C. albicans only (KP1), second group was fed with 50 mg/ day/ kg body weight aqueous extracts  from Neem leaves, then inoculated with C. albicans starting from day 8 until day 21 (KP2), third group was fed with 100mg/day/kg body weight aqueous extract from Neem leaves, then inoculated with C. albicans start from day 8 until day 21 (KP3),  fourth group was fed with 200 mg/day/kg body weight aqueous extract from Neem leaves, then inoculated by C. albicans start from day 8 until day 21 (KP4). The data was collected from swabbing the rat’s  tongue to calculate C. albicans colonies. The rats were acclimated and collected for immunohistochemistry measurement.  Results: The study showed that there were different result from ANOVA, HSD test, and linier regression. ANOVA showed  significant difference (p<0.01) between groups. The HSD test showed significant difference (p<0.05) between each groups. TNF- α was the stimuli sensor from environment, and used as parameter to see the effect from the change of innate immunity component to C. albicans. Conclusion:  Aqueous extract from neem leaves increased the macrophage TNF- α expression on C. albicans.

Keyword :

Neem leaves aqueous extract, Azadirachta Indica, macrophage, phagocytosis, Candida albicans,


References :

Ratna D,(2003) Daya hambat pertumbuhan C. albicans oleh perasan daun Mimba (Azadirachta Indica juss). Surabaya : Maj Ked Gigi (Dent J) Edisi khusus Temu Ilmiah Nasional III





Archive Article

Cover Media Content

Volume : 44 / No. : 1 / Pub. : 2011-03
  1. The Difference Of Plaque-index Between Blind And Deaf Children Before And After Dental Health Education
  2. Posterior Transverse Interarch Discrepancy On Hbe β Thalassemia Patients
  3. Cytotoxicity Study Of Reconstruction Plate Made Of Stainless Steel 316l Compared To Commercially Pure Titanium On Baby Hamster Kidney-21 Fibroblast Culture
  4. Calcium Hydroxide As Intracanal Dressing For Teeth With Apical Periodontitis
  5. Titanium – Ceramic Restoration: How To Improve The Binding Between Titanium And Ceramic
  6. Acupuncture Analgesia: The Complementary Pain Management In Dentistry
  7. Management Of Anterior Teeth Damage Caused By Complex Caries Through Aesthetic Endorestoration
  8. Changes Of The Sweet Taste Sensitivity Due To Aerobic Physical Exercise
  9. Cost Effectiveness And Quality Of Life Assessment On Dental Filling And Tooth Extraction In Balongsari Public Health Center
  10. Tnf- α Expression On Rats After Inoculated With Candida Albicans And Fed With Neem Extract (azadirachta Indica)
  11. Expression Of Matrix Metalloproteinase-8 Gene In Fixed Orthodontic Patients

Information DENTJ