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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 4 / Published : 2013-12

TOC : 10, and page :236 - 242

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Original Article :

Antifungal property of sticophus hermanii and holothuria atra extract and its cytotoxicityto gingiva-derived mesenchymal stem cell

Author :

  1. Kristanti Parisihni *1
  2. Syamsulina Revianti*2
  1. Department of Oral Biology Faculty of Dentistry,Universitas Hang Tuah Surabaya – Indonesia
  2. Department of Oral Biology Faculty of Dentistry,Universitas Hang Tuah Surabaya – Indonesia

Abstract :

Background: Sea cucumber had been acknowledged to have some medical propertiesSticophus hermanii and Holothuria atra are species of sea cucumber whichhas been known to have antifungal properties thus potentially explored as therapeutic agent in oral candidiasis. Purpose: The aim of this study is to evaluate the antifungal property Sticophus hermanii and Holothuria atra extractagainst Candida albicans and its cytotoxicity to human gingiva-derived mesenchymal stem cell.Methods: The study is an experimental laboratories research with post test only control groupdesign.Methanolic extract of Sticophus hermanii and Holothuria atra  in concentrations of 1%, 0.5%; 0.25%; 0.13%, 0.07%; 0.03%, 0.02% and 0.01%; were tested its cytotoxicity on gingiva-derived mesenchymal stem cell. Cell viability were measured  by MTT assay. The antifungal property against Candida albicanswas tested by disk diffusion method.Data were analyzed by ANOVA followed by LSD.Results: Extract of Sticophus hermanii showed no cytotoxicity in all concentrations (p>0.05), while Holothuria atra showed toxicity in the concentration of 1% and not cytotoxic in the concentrations below (p<0.05). Both sea cucumber extract could inhibit the growth Candida albicans, in vitro, proved by the clear zone around the disc in all concentrations (p<0.05). Conclusion: Stichopushermaniiand Holothuria atra extract had the antifungal effect against Candida albicans. Sea cucumber extract were not cytotoxic togingiva-derived mesenchymal stem cell in the concentration of Sticophus hermanii≤1% andHolothuria atra≤0.5%.   Keywords: 

Keyword :

Sticophus hermanii, Holothuria atra, cytotoxicity, gingiva-derived mesenchymalstem cell,


References :

  1. Mayer AMS , (2009). Marine pharmacology in 2005-6: marine compounds with anthelminthic, antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, antituberculosis, antiviral activities; affecting the cardiovascular immune and nervous system and other miscelanous mechanism of action. - : Biochim Biophys Acta


   


Archive Article

Cover Media Content

Volume : 46 / No. : 4 / Pub. : 2013-12
  1. Prevotella intermedia and porphyromonas gingivalis in dental caries with periapical granuloma
  2. Facial soft tissue profile on borderline class i malocclusion between extraction and non-extraction orthodontic treatment
  3. Ekspresi cox-2 setelah pemberian ekstrak etanolik kulit manggis
  4. Fish oil of sardinella longicep regulates osteoblast apoptosis of rat alveolar bone induced lypopolisacharide
  5. Inflammatory response of the dental pulp after application of 19% ethylene diamine tetraacetic acid and 37% phosphoric acid as etching agents
  6. Potential of extracts cassava leaf (manihot utilissima) on the expression of cox-2 on monocytes that exposed by lps e. coli
  7. The effect of desensitizing agent containing fluoride on streptococcus mutans adhesion and enamel roughness
  8. Physical properties of calcite synthesized hydroxyapatite as the filler of epoxy-resin-based root canal sealer
  9. Antifungal property of sticophus hermanii and holothuria atra extract and its cytotoxicityto gingiva-derived mesenchymal stem cell
  10. Orthodontic-surgical treatment of a severe class iii malocclusion
  11. Perbandingan korelasi antara indeks morfologi wajah genap dengan sudut interinsisal dan tinggi wajah secara sefalometri