UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 1 / Published : 2013-03

Order : 5, and page :18 - 22

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Original Article :

Antitumor activity of antisense oligonucleotide p45skp2 in soft palate carcinoma cell squamous in vitro

Author :

  1. Supriatno*1
  2. Sartari Entin Yuletnawati*2
  3. Iwa Sutardjo Rus Sudarso*3
  1. Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta - Indonesia
  2. Master course student , , Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta - Indonesia
  3. Department of Pedodontic, , Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta - Indonesia

Abstract :

Background: Human soft palate cancers are characterized by a high degree of local invasion and metastasis to the regional lymph nodes. Treatment options for this cancer are limited. However, a new strategy for refractory cancer, gene therapy is watched with keen interest. p45Skp2 gene as a tumor promoter gene is one of target of the oral cancer therapy. To inhibit the activity of p45Skp2 gene is carried-out the genetic engineering via antisense technique. Purpose: To examine the antitumor activity of p45Skp2 antisense (p45Skp2 AS) gene therapy in human soft palate [Hamakawa-Inoue (HI)] cancer cells. Methods: Pure laboratory experimental study with posttest only control group design was conducted as a research design. To investigate the apoptosis induction of p45Skp2 AStransfected cell was evaluated by colorimetric caspase-3 assay and Flow cytometry. Furthermore, to detect the suppression of in vitro HI cell invasion and cell growth of p45Skp2 AS-treatment cell was examined by Boyden chamber kit and MTT assay, respectively. Results: The cell number of p45Skp2 AS-treated HI cell was significant decreased when compared with that of p45Skp2 sense (p45Skp2 S) cells (p<0.05). p45Skp2 AS-treated cell induced apoptosis characterized by an increase in the early and late apoptosis, and activation of caspase-3 (p<0.05). Therefore, suppression of HI cell invasion and cell growth were markedly increased by p45Skp2 AS treatment (p<0.05). Conclusion: Antisense oligonucleotide p45Skp2 has a high antitumor activity in human soft palate cancer cell, targeting this molecule could represent a promising new therapeutics approach for this type of cancer.

Keyword :

Antisense p45Skp2 gene, apoptosis, invasion, soft palate cancer cell,


References :

Cohan DM,(2009) Oropharyngeal cancer: current understanding and management - : . Curr Opin Otolaryngol Head Neck Surg





Archive Article

Cover Media Content

Volume : 46 / No. : 1 / Pub. : 2013-03
  1. Patient’s Expectation On Communication Performances Community Of Dental Health Services Providers Located In Urban And Rural Area
  2. Changes In Setting Time Of Alginate Impression Material With Different Water Temperature
  3. Bactericidal And Cytotoxic Effects Of Erythrina Fusca Leaves Aquadest Extract
  4. The Role Of Inducible Nitric Oxide Synthase In Teeth Periapical Lesions Immunopathogenesis Caused By Enterococcus Faecalis
  5. Antitumor Activity Of Antisense Oligonucleotide P45skp2 In Soft Palate Carcinoma Cell Squamous In Vitro
  6. Evaluation Of Local Muscle Soreness Treatment With Anterior Bite Splint Made Of Soft Putty Impression Material
  7. Unidentified Angular Recurrent Ulceration Responsive To Antiviral Therapy
  8. The Inhibition Of Malignant Epithelial Cells In Mucosal Injury In The Oral Cavity Of Strains By Pomegranate Fruit Extract (punica Granatum Linn) Through Bcl-2 Expression
  9. Shear Strength Of Orthodontic Bracket Bonding With Gic Bonding Agent After The Application Of Cpp-acpf Paste
  10. Hemolysin Activities As Virulence Factor Of Enterococcus Faecalis Isolated From Saliva And Periapical Abscess (gene Detection By Pcr)
  11. Effect Of Citrus Aurantifolia Swingle Essential Oils On Methyl Mercaptan Production Of Porphyromonas Gingivalis Triggering Halitosis

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