UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 4 / Published : 2012-12

Order : 4, and page :197 - 201

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Original Article :

Antitumor activity of intratumoral injection of pcdna3.1-p27kip1mt followed by in vivo electroporation in a malignant burkitt’s lymphoma cell xenograft

Author :

  1. Supriatno*1
  2. Sartari Entin Yuletnawati*2
  1. Department of Oral Medicine, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta - Indonesia
  2. Postgraduate Student, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta - Indonesia

Abstract :

Background: Human malignant Burkitt’s lymphomas are an uncommon type of Non-Hodgkin Lymphoma commonly affects in children. It is a highly aggressive type of B-cell lymphoma. Treatment for this malignant are still limited. However, a new strategy for refractory cancer, gene therapy is watched with keen interest. Recently, a novel method for high-efficiency and region-controlled in vivo gene transfer was developed by combining in vivo electroporation and plasmid cDNA. In the present study, a non-viral gene transfer system, in vivo electroporation in human malignant Burkitt’s lymphoma (Raji) cell xenograft was investigated. Purpose: The purpose of this study was to evaluate p27Kip1 gene therapy in Raji cell xenografts using pcDNA3.1-p27Kip1 mutant type (mt) and pcDNA3.1 empty vector (neo) with the local application of electric pulses. Methods: True experimental study using post-intervention with control group design was performed in this study. Material sample was obtained from integrated research laboratory at faculty of dentistry, Gadjah Mada University, Yogyakarta. The efficiency of transfection of exogenous p27Kip1 gene by electroporation was confirmed by Western bloting analysis. To evaluate the reduction of malignant Burkitt’s lymphoma cell xenografts by this method, the volume of Raji cell xenografts in mice after electroporation with p27Kip1 mt or neo gene was measured. Results: Up-regulation of p27Kip1 protein was detected in pcDNA3.1-p27Kip1 mt. Furthermore, the growth of tumors was markedly suppressed by p27Kip1 mt gene transfection compared with transfection of neo. Conclusion: Injection of pcDNA3.1-p27Kip1 mt gene followed by in vivo electroporation has a high-potentially to suppress the growth of malignant Burkitt’s lymphoma cells. Furthermore, combination system of pcDNA3.1-p27Kip1 mt-injected tumor and electroporation might be used for human oral cancer.

Keyword :

mutant type p27Kip1, human malignant Burkitt’s lymphoma, electroporation,


References :

Supriatno,(2011) Effect of intratumoral injection of mutant type p27Kip1 followed by in vivo electroporation on radiotherapy-resistant human oral tongue cancer xenografts - : Mol Med Report





Archive Article

Cover Media Content

Volume : 45 / No. : 4 / Pub. : 2012-12
  1. The Role Of Actinobacillus Actinomycetemcomitans Fimbrial Adhesin On Mmp-8 Activity In Aggressive Periodontitis Pathogenesis
  2. Aesthetic Treatment On Anterior Teeth Crown Fracture Caused By Dental Trauma
  3. Molecular Characterization Of Interleukin-1a (+4845) Gtgene In Aggresive Periodontitis Patients
  4. Antitumor Activity Of Intratumoral Injection Of Pcdna3.1-p27kip1mt Followed By In Vivo Electroporation In A Malignant Burkitt’s Lymphoma Cell Xenograft
  5. The Effect Of Nickel As A Nickel Chromium Restoration Corrosion Product On Gingival Fibroblast Through Analysis Of Bcl-2
  6. Inhibition Of Dental Plaque Formation By Toothpaste Containing Propolis
  7. Inhibition Effect Of Cashew Stem Bark Extract (anacardium Occidentale L.) On The Inhibition Of Biofilm Formation Of Streptococcus Sanguinis
  8. Antibacterial Efficacy Of Salvadora Persicaas A Cleansing Teeth Towards Streptococcus Mutansand Lactobacilli Colonies
  9. Pomegranate Juice As An Ideal Mouthrinse For Fixed Orthodontic Patients
  10. The Increasing Of Fibroblast Growth Factor 2, Osteocalcin, And Osteoblast Due To The Induction Of The Combination Of Aloe Vera And 2% Xenograft Concelous Bovine
  11. The Ph Changes Of Artificial Saliva After Interaction With Oral Ph Changes Of Artificial Saliva After Interaction With Oral Changes Of Artificial Saliva After Interaction With Oral Micropathogen

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