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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 1 / Published : 2003-03

Order : 3, and page :9 - 13

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Original Article :

Bactericidal and cytotoxic effects of erythrina fusca leaves aquadest extract

Author :

  1. Janti Sudiono*1
  2. Ferry Sandra*2
  3. Nadya Saputri Halim*3
  4. Timotius Andi Kadrianto*4
  5. Melinia*5
  1. Department of Oral Pathology, Faculty of Dentistry, Universitas Trisakti, Jakarta - Indonesia
  2. Department of Biochemistry, Faculty of Dentistry, UniversitasTrisakti, Jakarta-Indonesia
  3. Department of Biochemistry, Faculty of Dentistry, UniversitasTrisakti, Jakarta-Indonesia
  4. Department of Biochemistry, Faculty of Dentistry, UniversitasTrisakti, Jakarta-Indonesia
  5. Dental Practitioner, Jakarta - Indonesia

Abstract :

Background: Empirically, Erythrina fusca has been used as traditional herb for its antibacterial and antiinflammation properties. Periodontal disease is one of the most oral infectious diseases with microorganism predominated as the contributing factors. Porphyromonas gingivalis (P. gingivalis) is one of the main bacteria pathogen found in periodontal diseases. Purpose: The purpose of this study was to examine the bactericidal effect on P. gingivalis and cytotoxic effect on fibroblast of Erythrina fusca Leaves Aquadest Extract (EFLAE) at various concentrations. Methods: Pure P. gingivalis was cultured in Brain Heart Infusion (BHI) medium for 24 hours with or without various concentrations of treatment of EFLAE. Calculation and statistical analysis of remaining bacteria were performed by inhibitory zone method to evaluate the EFLAE bactericidal effect and compared to chlorhexidine as positive control. To evaluate the cytotoxic effect, NIH 3T3 cells were cultured in Dulbecco’s Modification of Eagle’s Medium (DMEM) containing of 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, pH 7.2, in 5% CO2, and stored in humidified incubator under temperature 370 C. Cells were treated with/without various concentrations of EFLAE for 48 hours. The viable cells were then counted using 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazodium bromide (MTT) method. Results: EFLAE have bactericidal effect on P. gingivalis in a concentration dependent manner starting from 78%. The concentration of 90% EFLAE had stronger bactericidal effect (35.004 ± 1.546) than those of chlorhexidine as positive control (32.313 ± 1.619). One-way ANOVA showed significant bactericidal effect differences among concentrations of EFLAE and chlorhexidine (p<0.05) while Tuckey HSD test showed significant difference only between lower concentration of EFLAE (78%, 79%) and chlorhexidine. With the highest concentration of EFLAE (100%) applied in the bactericidal test, no cytotoxic effect of EFLAE on NIH 3T3 cells was detected. Conclusion: EFLAE could inhibit the growth of P. gingivalis in a concentration dependent manner, starting from 78%. There was no evidence of EFLAE’s cytotoxic effect on fibroblast.

Keyword :

EFLAE, bactericidal, citotoxicity,


References :

Valkenburg JLCH ,(2001) Plant resources of South- East Asia: Medicinal and Poisonous Plants 2. Backhuys Publishers : Leiden





Archive Article

Cover Media Content

Volume : 46 / No. : 1 / Pub. : 2013-03
  1. Patient’s Expectation On Communication Performances Community Of Dental Health Services Providers Located In Urban And Rural Area
  2. Changes In Setting Time Of Alginate Impression Material With Different Water Temperature
  3. Bactericidal And Cytotoxic Effects Of Erythrina Fusca Leaves Aquadest Extract
  4. The Role Of Inducible Nitric Oxide Synthase In Teeth Periapical Lesions Immunopathogenesis Caused By Enterococcus Faecalis
  5. Antitumor Activity Of Antisense Oligonucleotide P45skp2 In Soft Palate Carcinoma Cell Squamous In Vitro
  6. Evaluation Of Local Muscle Soreness Treatment With Anterior Bite Splint Made Of Soft Putty Impression Material
  7. Unidentified Angular Recurrent Ulceration Responsive To Antiviral Therapy
  8. The Inhibition Of Malignant Epithelial Cells In Mucosal Injury In The Oral Cavity Of Strains By Pomegranate Fruit Extract (punica Granatum Linn) Through Bcl-2 Expression
  9. Shear Strength Of Orthodontic Bracket Bonding With Gic Bonding Agent After The Application Of Cpp-acpf Paste
  10. Hemolysin Activities As Virulence Factor Of Enterococcus Faecalis Isolated From Saliva And Periapical Abscess (gene Detection By Pcr)
  11. Effect Of Citrus Aurantifolia Swingle Essential Oils On Methyl Mercaptan Production Of Porphyromonas Gingivalis Triggering Halitosis

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