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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 43 / No. 4 / Published : 2010-12

Order : 4, and page :176 - 180

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Original Article :

Biocompatibility and osteoconductivity of injectable bone xenograft, hydroxyapatite and hydroxyapatite-chitosan on osteoblast culture

Author :

  1. Bachtiar EW *1
  2. Bachtiar BM *2
  3. Abas B *3
  4. Harsas NA *4
  5. Sadaqah NF*5
  6. Aprilia R *6
  1. Department of Oral Biology, Faculty of Dentistry, University of Indonesia
  2. Department of Oral Bilogy, Faculty of Dentistry, University of Indonesia Jakarta - Indonesia
  3. Badan Tenaga Nuklir Nasional (BATAN) Jakarta - Indonesia
  4. Department of Oral Bilogy, Faculty of Dentistry, University of Indonesia Jakarta - Indonesia
  5. Department of Oral Biology, Faculty of Dentistry, University of Indonesia
  6. Department of Oral Biology, Faculty of Dentistry, University of Indonesia

Abstract :

Background: Bone graft in the form of injectable paste gives several advantages over the powder form as it could be placed in the defect area that has limited accessibility. Purpose: The purpose of this study was to assess biocompatibility and osteoconductivity of an injectable bone xenograft (IBX), injectable hydroxyapatite (IHA) and injectable hydroxyapatite-chitosan (IHA-C) on osteoblastic cell line (MG-63). Methods: Three concentrations (0.25%, 0.5% and 1.0%) of IBX, IHA and IHA-C were supplemented with DMEM culture medium. The viability cells were measured by MTT assay 4 hour after incubation. ALP activity was measured at day 1, 3, 5 and 7. Calcium deposition was tested at day 3 and day 7 by means of Von Kossa staining. Results: MTT assay showed that the viability cells of all the test groups were above 100% compared to the control group. The cell viability of the 0.25% IHA paste was significantly higher (115.02% ± 4.37%, p < 0.05) compared with IBX paste and IHA-C in all concentrations tested. The highest level of ALP secretion of all test groups was found on the fifth day of exposure. The highest level of ALP in the IBX paste group was 0.25% concentration while the highest level of ALP in the IHA-C and IHA paste group was 1% and 0.25%, respectively. In addition, the highest calcium deposition was shown on IHA 1% at day 7 (p > 0.05). Conclusion: It was suggested that adequate biocompatibility and osteoconductivity was evident for all injectable pastes tested.

Keyword :

Injectable bone xenograft, injectable hydroxyapatite, injectable hydroxyapatite-chitosan, osteoblast,


References :

Garg AK ,(1999) Tissue engineering: Applications in maxillofacial surgery and periodontics Illnois : Quintessence Public Inc





Archive Article

Cover Media Content

Volume : 43 / No. : 4 / Pub. : 2010-12
  1. Clinical Consideration Of Thrombocytopenia In Children
  2. The Increasing Of Odontoblast-like Cell Number On Direct Pulp Capping Of Rattus Norvegicus Using Chitosan
  3. Minor Modification Of Millard Surgical Technique For Correction Of Complete Unilateral Cleft Lip
  4. Biocompatibility And Osteoconductivity Of Injectable Bone Xenograft, Hydroxyapatite And Hydroxyapatite-chitosan On Osteoblast Culture
  5. Alveolar Ridge Rehabilitation To Increase Full Denture Retention And Stability
  6. Pulp Nerve Fibers Distribution Of Human Carious Teeth: An Immunohistochemical Study
  7. The Management Of Dental Fracture On Tooth 61 In A Child With Attention Deficit Hyperactivity Disorders
  8. Multidisciplinary Management Of A Mandibular Buccal Plate Perforation
  9. Candida Albicans Adherence On Acrylic Resin Plates Immersed In Black Tea Steeping
  10. A Combination Of Endodontic Therapy And Root Resection In Furcation Involvement Case
  11. Interleukin-1β Expression Among Periodontitis Patients In Surabaya

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