UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 2 / Published : 2013-06

Order : 11, and page :108 - 113

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Original Article :

Bitemark identification with chelex methods in dna extraction

Author :

  1. Imelda Kristina Sutrisno*1
  2. Ira Arundina*2
  3. Agung Sosiawan*3
  1. Mahasiswa Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: In the case of crime often encountered evidence in bitemark form that was found on the victim’s body. Generally, to identify bitemark use standard techniques that compare the interpretation photo with the tooth model of suspected person. However in the fact this technique did not get an accurate results. Therefore we need another technique to identify the bitemark case for example is with irrigating the bitemark to get a sample of saliva and the remaining epithelium attached so we can identify the DNA from the person. In this processes, we often find a limited material, not only less in quantity but also less in quality, so we need an effective method of extracting DNA,that is called Chelex method. Purpose: Proving the success of DNA extraction with Chelex method on a sample bitemark. Methods: Five persons with blood and bitemark were taken, each sampel was exctracted with Chelex and then tested the quantity with UV Spechtrophotometer. After that the results was amplified by PCR at locus vWA and TH01 then it was vizualised by electrophoresis. Results: The elektrophoresis’s results shows band at locus vWA and TH01 for blood sample and bitemark with no significant differences in the which mean the Chelex method can be used for bitemark. Conclusion: It was proven that Chelex method can be use to extract DNA from bitemark.

Keyword :

Bitemark, Chelex, Locus vWA , TH01,


References :

van der Velden A,(2006) Bitemark analysis and comparison using image perception and technology - : J Forensic Odontostomatol





Archive Article

Cover Media Content

Volume : 46 / No. : 2 / Pub. : 2013-06
  1. Enamel Defect Of Primary Dentition In Sga Children In Relation To Onset Time Of Intrauterine Growth Disturbance
  2. Description Of Pulp Chamber Density In Deciduous Teeth Using Cone Beam Ct-3d
  3. A Study Of Extraction And Characterization Of Alginates Obtained From Brown Macroalgae Sargassum Duplicatum And Sargassum Crassifolium From Indonesia
  4. Effectiveness Of Various Sterilization Methods Of Contaminated Post-fitted Molar Band
  5. Peroxide Alkaline For Cleansing The Baby Bottle Nipple To Prevent Oral Thrush Relaps
  6. Treatment Of Non-vital Primary Molar Using Lesion Sterilization And Tissue Repair (lstr 3mix-mp)
  7. New Concept In Allergy: Non-allergic Rats Becomes Allergic After Induced By Porphyromonas Gingivalis Lipopolysaccharide
  8. Ricketts Esthetic Line At Student Of Faculty Of Dentistry Universitas Airlangga
  9. Dental Student’s Perception To Aesthetic Component Of Iotn And Demand For Orthodontic Treatment
  10. Compressive Strength Of Hybrid Composites Resin With Post Curing Light Emitting Diode Using Three Different Sizes Of Lightbox
  11. Bitemark Identification With Chelex Methods In Dna Extraction

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