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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 3 / Published : 2014-09

Order : 5, and page :141 - 145

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Original Article :

Characterization of lactoferrin in gingival crevicular fluid of chronic periodontitis patient

Author :

  1. Sisca Meida Wati*1
  2. Istiati*2
  3. Pratiwi Soesilawati*3
  1. Dosen Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: Human periodontal diseases are inflammatory disorders that are the result of complex interactions between periodontopathogens and the host’s immune response. Periodontitis results in tooth loss and can even lead to diseases of the whole body if not treated. Gingival crevicular fluid (GCF) reflects the condition of the gingiva and contains proteins transuded from serum or cells at inflamed sites. Polymorphonuclear leukocyte (PMNs) infiltration can be seen in each stage of periodontitis. Lactoferrin is one of the PMN specific granules and could be a useful marker of PMN activity. Purpose: The aim of this study was to determine the band intensity of lactoferrin used as periodontitis biomarker. Methods: Gingival crevicular fluid (GCF) samples were collected using paper point no.30 from 40 subjects, 30 periodontitis patients that devide according to the severity (10 mild periodontitis, 10 moderate periodontitis, and 10 severe periodontitis) and 10 healthy controls, ranging in ages from 20 to 35 years. GCF lactoferrin was analyzed by Western blot and measured the band intensity by quantity one software (bio-rad). results:The periodontitis sites exhibited significantly greater band intensity of lactoferrin than healthy sites. The band intensity of lactoferrin was positively correlated with the severity of periodontitis (α = 0.05). Conclusion: The results of this study showed that the intensity of the lactoferrin protein bands could be used as biomarkers of periodontitis. 

Keyword :

Gingival crevicular fluid, lactoferrin, periodontitis, band intensity,


References :

Kuula H ,(2009) Local and systemic responses in matrix metalloproteinase 8-deficient mice during Porphyromonas gingivalis-induced periodontitis - : Infect Immun





Archive Article

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Volume : 47 / No. : 3 / Pub. : 2014-09
  1. Microscopic Evaluation Of The Interface Between Various Of Glass Ionomer And Dentin Surface: Influence Of Nanoparticle Horseshoe Crab (tachypleus Gigas) Chitosan Addition
  2. Modulation Of Fgf2after Topical Application Ofr Topical Application Of Topicalapplication Ofapplication Of�tichopus Hermaniigelontichopus Hermaniigelon Traumatic Ulcer In Wistar Rats
  3. Comparison Of Three Types Algyrogel Cytotoxicity Against Fibroblasts
  4. The Effect Of Aspirin Administration Length On Ki-67 Expression Protein And Oral Epithelial Mucosal Thickness In Male Wistar Mice
  5. Characterization Of Lactoferrin In Gingival Crevicular Fluid Of Chronic Periodontitis Patient
  6. Oral Health Knowledge Among Parents Of Autistic Child In Bandung-indonesia
  7. Effect Of Siwak (salvadora Persica) Traditional Toothbrush On The Accumultated Dental Plaque
  8. The Management Of Impacted Permanent Canine With Surgical Exposure
  9. Inhibition Effect Of Xylitol And Nistatin Combination On Candida Albicans Growth (in Vitro)
  10. The Release Of Nickel And Chromium Ions In Special Type Of Australian Wire And Stainless Steel In Artificial Saliva
  11. Identifikasi Bakteri Saat Insial Akuisisi Streptococcus Mutans Pada Bayi

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