UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 3 / Published : 2014-09

Order : 3, and page :130 - 134

Related with : Scholar   Yahoo!   Bing

Original Article :

Comparison of three types algyrogel cytotoxicity against fibroblasts

Author :

  1. Stefany Elan Saktiyawardani*1
  2. Hardono Jaya Lauson*2
  3. Anugerah Pekerti Astamurtiningrum*3
  4. Mahadna Aulia Rahmah*4
  5. Pramana Pananja Putra*5
  6. Juni Handajani*6
  1. agian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
  2. Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
  3. Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
  4. Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada
  5. Fakultas Kedokteran, Universitas Gadjah Mada
  6. Bagian Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Abstract :

Background: The prevalence of osteoporosis and bone defects in the world and Indonesia getting more increased each year. Algyrogel is a alginate-based hydrogel material lately very popular used as a bone substitute through Injectable Bone Substitute (IBS) method. One of advantages algyrogel is biocompatibility to the body tissues. Some natural-based polymer (alginate, chitosan,  and hyaluronat) have different biocompatibility. Purpose:Compare the cytotoxicity of three types algyrogel against fibroblast cells. Methods: This study used three types algyrogel, there were algyrogel containing alginate (Alg), a mixture of alginate with chitosan (Alg/Ch), and a mixture of alginate with hyaluronic acid (Alg/Ha). Each type algyrogel was exposed in the Vero cell line of fibroblast with volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; and 20 μL. The cytotoxicity performed by MTT method to determine the percentage of cell death. The optical density was measured by an ELISA plate reader then data was analyzed using ANOVA and probit. Results:The percentage of cell death in all groups of algyrogel have below 30%. LC50 value at Alg is 651.017 mg/mL (21.56%), Alg/Ch is 280.478 mg/mL (20.91%), and Alg/Ha is 1054.094 mg/ mL (17.2%). Conclusion: Alg/Ha has the lowest cytotoxicity on fibroblast cells and may have potential as an osteoconductor-synthetic bone product. 

Keyword :

Algyrogel, cytotoxicity, fibroblasts, bone synthesis,


References :

Morais DS ,(2013) Biological evaluation of alginate-based hydrogels, with antimicrobial features by Ce(III) incorporation, as vehicles for a bone substitute - : J Mater Sci Mater Med





Archive Article

Cover Media Content

Volume : 47 / No. : 3 / Pub. : 2014-09
  1. Microscopic Evaluation Of The Interface Between Various Of Glass Ionomer And Dentin Surface: Influence Of Nanoparticle Horseshoe Crab (tachypleus Gigas) Chitosan Addition
  2. Modulation Of Fgf2after Topical Application Ofr Topical Application Of Topicalapplication Ofapplication Of�tichopus Hermaniigelontichopus Hermaniigelon Traumatic Ulcer In Wistar Rats
  3. Comparison Of Three Types Algyrogel Cytotoxicity Against Fibroblasts
  4. The Effect Of Aspirin Administration Length On Ki-67 Expression Protein And Oral Epithelial Mucosal Thickness In Male Wistar Mice
  5. Characterization Of Lactoferrin In Gingival Crevicular Fluid Of Chronic Periodontitis Patient
  6. Oral Health Knowledge Among Parents Of Autistic Child In Bandung-indonesia
  7. Effect Of Siwak (salvadora Persica) Traditional Toothbrush On The Accumultated Dental Plaque
  8. The Management Of Impacted Permanent Canine With Surgical Exposure
  9. Inhibition Effect Of Xylitol And Nistatin Combination On Candida Albicans Growth (in Vitro)
  10. The Release Of Nickel And Chromium Ions In Special Type Of Australian Wire And Stainless Steel In Artificial Saliva
  11. Identifikasi Bakteri Saat Insial Akuisisi Streptococcus Mutans Pada Bayi

Information DENTJ