UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 1 / Published : 2012-03

Order : 8, and page :39 - 42

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Original Article :

Crude toxin of aggregatibacter actinomycetemcomitans serotype-b increase parp-1 expression in gingival epithelium

Author :

  1. Ernie Maduratna Setiawatie*1
  1. Department of Periodontics/ITD Researcher Faculty of Dentistry, Airlangga University Surabaya - Indonesia

Abstract :

Background: Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitants) serotype-B has long been associated with aggressive periodontitis. Gingival epithelial cell is exquisitely sensitive to the toxin so that may lead to disruption of the epithelial protective barrier, facilitating invasion and perturbation of the underlying connective tissue. Currently suggested that Aa serotype-B produce protein toxin that caused DNA strand breaks. PARP-1 is an abundant nuclear protein functioning as a DNA nick-sensor enzyme. PARP-1 was one of the first identified substrates of caspases, the main executioners of apoptosis. Therefore, a role for PARP-1 in the regulation of apoptosis has been suggested. Purpose: The purpose of this study was to prove PARP-1 expression in gingival epithelium caused by toxin exposure of A. actinomycetemcomitant serotype-B. Methods: This is an experimental study involving twenty adult mice strain Swiss Webster (balb C) divided randomly into two groups: control group (Group A) and toxin group (Group B). Both group were acclimated for one week before treatment. Group A was applied topically with sterile distillated water every 12 hours. Group B was applied topically by 100μg/ml of crude toxin A. actinomycetemcomitant serotype B at the buccal area of mandibular anterior teeth using Hamilton syringe. The mice were sacrificed at 24 hours after toxin application, and then the tissue sections of gingival epithelium were stained with immunohistochemistry to reveal the PARP-1 expression. The data were analyzed with t-test. Results: The PARP-1 expression exhibited an increase with the toxin group (mean= 48.9; SD= 2.01) compared with the control group (mean= 25.21; SD= 1.72). DNA fragmentation appeared from the agarose gel examination, marked as DNA laddering, indicate the cell apoptosis. Conclusion: In conclusion the crude toxin exposure of A. actinomycetemcomitant serotype-B leads to DNA fragmentation and increase PARP-1 expression.

Keyword :

Crude toxin of Aggregatibacter actinomycetemcomitans serotype-B, PARP-1, DNA fragmentation,


References :

Daniel HF,(2007) A. actinomycetemcomitansand its relationship to initiation of localized aggressive periodontitis - : J Clin Microbiol





Archive Article

Cover Media Content

Volume : 45 / No. : 1 / Pub. : 2012-03
  1. Immediate Overdenture For Improving Aesthetic Of Anterior Teeth With Periodontal Problem
  2. Orthodontic Treatment Considerations In Down Syndrome Patients
  3. The Effective Concentration Of Red Betel Leaf (piper Crocatum) Infusion As Root Canal Irrigant Solution
  4. Degrees Of Chitosan Deacetylated From White Shrimp Shell Waste As Dental Biomaterials
  5. Antimicrobial Effect Of Chlorine Dioxide On Actinobacillus Actinomycetemcomitans In Diabetes Mellitus Rats Treated With Insulin
  6. Genetic Variability Of Candida Albicans In Hiv/aids Patient With And Without Arv Therapy And Non Hiv/aids
  7. Relationship Between Salivary Fluor Concentration And Caries Index In 12–15 Years Old Children
  8. Crude Toxin Of Aggregatibacter Actinomycetemcomitans Serotype-b Increase Parp-1 Expression In Gingival Epithelium
  9. Effectiveness Of Bleaching Agent On Composite Resin Discoloration
  10. Analysis Of Importance Level And Quality Achievement Aspect In Dental Health Service (a Case Study On Waru Sidoarjo Community Dental Health Service)
  11. Effect Of Robusta Coffee Beans Extract Ointment On Full Thickness Wound Healing

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