UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 38 / No. 1 / Published : 2005-01

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Original Article :

Cytotoxicity of the cyanoacrylate restoration material with variation of powder and liquid ratio by using mtt assay

Author :

  1. Asti Meizarini*1
  1. Department of Dental Material, Faculty of Dentistry Airlangga University, Surabaya - Indonesia

Abstract :

The requirements for dental material include not toxic, not irritant, no carcinogenic potential, nor cause an allergic response with the use in oral cavity. The cyanoacrylate restoration material has certain substance that can be toxic. Because of the ratio amount of powder and liquid is not known, it can lead the restoration more toxic. The purpose of this study was to know the cytotoxicity of the cyanoacrylate restoration material with different variation of powder and liquid ratio using MTT assay. Six cylinder samples of 5 mm in diameter and 2 mm in thickness were used for each group of 1:1.00; 1:0.75; 1:0.50 powder and liquid ratio of cyanoacrylate restoration materials. Each of samples was immersed in eppendorf micro tubes consisting of media culture. After 24 hour, the immersion of media culture was used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. The density of optic formazan indicated the amount of living cells. All data were statistically analyzed by one-way ANOVA and HSD. The results showed that the percentage of living cells amount of powder and liquid ratio 1:1.00; 1:0.75; 1:0.50 were 98.59%; 95.76%; 94.92% respectively. There was a significant difference between 1:1.00 and 1:0.50 group ratio. The conclusion was that the cytotoxicity between 1:1.00 and 1:0.50 powder and liquid ratio of cyanoacrylate restoration materials in this study decreased.

Keyword :

cytotoxicity, cyanoacrylate restoration material, powder and liquid ratio, MTT assay ,


References :

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MF



Archive Article

Cover Media Content

Volume : 38 / No. : 1 / Pub. : 2005-03
  1. The Effect Of Increasing Sucrose Concentration In Diet Toward The Content Of Calcium In Tooth Of Wistar Rats
  2. The Adherence Of Streptococcus Mutans Colony To Surface Visible Light Composite Resins
  3. Stimulating Factor Of Parents' Motivation To Take Their Children's Dental Health For Treatment In The Faculty Of Dentistry Airlangga University
  4. The Transverse Strength Of The Hybrid Acrylic Resin After Glass Fiber Reinforcement With Different Method
  5. Cytotoxicity Of The Cyanoacrylate Restoration Material With Variation Of Powder And Liquid Ratio By Using Mtt Assay
  6. The Role Of Sorbitol In Maintaining Saliva’s Ph To Prevent Caries Process
  7. Shear Bond Strenght Of Glass Ionomer Cement In Dentin And Nicr Alloy
  8. Effects Of Materials Thickness And Length Of Light Exposure On The Surface Hardness Light-cured Composite Resins
  9. The Color Changes Of Acrylic Resins Denture Base Material Which Are Immersed In Sodium Hypochlorite And Chlorhexidine
  10. The Role Of Music As A Dental Practice Facility In Reducing Patient’s Anxiety
  11. The Comparison Of The Antibacterial Effect Of Irrigation Solutions Hydrogen Peroxyde 3% And Piper Betle Folium Infusum 20% To Bacterial Mix
  12. The Advantage Of The Three Dimensional Computed Tomographic (3 D-ct) For Ensuring Accurate Bone Incision In Sagittal Split Ramus Osteotomy

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