UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 41 / No. 3 / Published : 2008-07

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Original Article :

Cytotoxicity test of 40, 50 and 60% citric acid as dentin conditioner by using mtt assay on culture cell line

Author :

  1. Christian Khoswanto*1
  2. Ester Arijani*2
  3. Pratiwi Soesilawati*3
  1. Department of Oral Biology
    Faculty of Dentistry Airlangga University
    Surabaya - Indonesia
  2. Department of Oral Biology
    Faculty of Dentistry Airlangga University
    Surabaya - Indonesia
  3. Department of Oral Biology
    Faculty of Dentistry Airlangga University
    Surabaya - Indonesia

Abstract :

Background: Open dentin is always covered by smear layer, therefore before restoration is performed, cavity or tooth which has been prepared should be clean from dirt. The researchers suggested that clean dentin surface would reach effective adhesion between resin and tooth structure, therefore dentin conditioner like citric acid was used to reach the condition. Even though citric acid is not strong acid but it can be very erosive to oral mucous. Several requirements should be fulfilled for dental product such as non toxic, non irritant, biocompatible and should not have negative effect against local, systemic or biological environment. Cytotoxicity test was apart of biomaterial evaluation and needed for standard screening. Purpose: This study was to know the cytotoxicity of 40, 50, 60% citric acid as dentin conditioner using MTT assay. Method: This study is an experimental research using the Post-Test Only Control Group Design. Six samples of each 40, 50 and 60% citric acid for citotoxicity test using MTT assay. The density of optic formazan indicated the number of living cells. All data were statistically analyzed by one way ANOVA. Rresult: The percentage of living cells in 40, 50 and 60% citric acid were 95.14%, 93.42% and 93.14%. Conclusion: Citric acid is non toxic and safe to be used as dentine conditioner.

Keyword :

cytotoxicity, citric acid, MTT assay,


References :





Archive Article

Cover Media Content

Volume : 41 / No. : 3 / Pub. : 2009-09
  1. Cytotoxicity Test Of 40, 50 And 60% Citric Acid As Dentin Conditioner By Using Mtt Assay On Culture Cell Line
  2. Cytotoxicity Of 5% Tamarindus Indica Extract And 3% Hydrogen Peroxide As Root Canal Irrigation
  3. The Effectiveness Of 0.5–0.7% Tetracycline Gel To Reduced Subgingival Plaque Bacteria
  4. The Profile Of Upper Integument Lip Of Baduy And The Nearby Living Sundanese In South Banten, West Java, Indonesia
  5. Isolation And Identification Of Java Race Amniotic Membrane Secretory Leukocyte Protease Inhibitor Gene
  6. The Role Of Proper Treatment Of Maxillary Sinusitis In The Healing Of Persistent Oroantral Fistula
  7. The Management Of Oral Candidosis In Diabetic Patient With Maxillary Herpes Zoster
  8. Non-invasive Endodontic Treatment Of Large Periapical Lesions
  9. Potential Role Of Odontoblasts In The Innate Immune Response Of The Dental Pulp
  10. The Use Of Bay Leaf (eugenia Polyantha Wight) In Dentistry
  11. Effect Of Various Temperature And Storage Duration On Setting Time Of Orega Sealer

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