Dental Journal (Majalah Kedokteran Gigi)
ISSN 1978-3728
Vol. 45 / No. 1 / Published : 2012-03
Order : 4, and page :17 - 21
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Original Article :
Degrees of chitosan deacetylated from white shrimp shell waste as dental biomaterials
Author :
- Sularsih *1
- Anita Yuliati*2
- Coen Pramono Danudiningrat*3
- Department of Dental Material, Faculty of Dentistry, Hang Tuah University
- Department of Dental Material, Faculty of Dentistry, Airlangga University, Surabaya - Indonesia
- Department of Oral and Maxillofacial Surgery Faculty of Dentistry, Airlangga University Surabaya - Indonesia
Abstract :
Background: Chitosan is biomaterial improved for various dentistry applications because it is biocompatible, degradable, nontoxic, and not carcinogenic. The main parameter affecting the characteristics of chitosan is deacetylation degree. Purpose: This study is aimed to determine the degree of deacetylated of chitosan derived from white shrimp shell waste used as dental biomaterial. Methods: White shrimp shells were crushed into powder. Next, deproteination process was conducted with 3.5% NaOH solution, demineralized with 1N HCl solution, and then depigmented with 90% acetone solution into chitin powder. Deacetylation process was then conducted by soaking the chitin powder in 50% NaOH solution for 6 h at 65° C to produce white powder of chitosan. Afterwards, deacetylation degree test was conducted by using Fourier Transform Infrared Spectrophotometer (FTIR) to calculate the ratio of the absorption bands between the absorbance peak of amide group about 1655 cm-1 and the absorbance peak of hydroxyl group about 3450 cm-1. Results: The result of the deacetylation degree test on the chitosan powder derived from white shrimp shell waste was high, about 85.165%, and had the eligible form, solubility, and pH. Conclusion: It can be concluded that the deacetylation degree of chitosan from white shrimp shells could reach 85.165%.
Keyword :
Chitosan, shrimp shell waste, deacetylation degree,
References :
Maretaningtias DA,(2009) Toxicity testing of chitosan from tiger prawn shell wate on cell culture surabaya : Maj Kedokteran Gigi (Dent J )
Archive Article
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Volume : 45 / No. : 1 / Pub. : 2012-03 |
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