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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 3 / Published : 2013-09

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Original Article :

Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ

Author :

  1. Nurdiana Dewi*1
  2. Ahmad Syaify*2
  3. Ivan Arie Wahyudi*3
  1. Study Program of Dental Science, Medical Faculty, Universitas Lambung Mangkurat, Banjarmasin – Indonesia
  2. Departement of Periodontology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta – Indonesia
  3. Departement of Biomedica, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta – Indonesia

Abstract :

Background: Amelogenin is a major protein constituent of the developing enamel matrix that is critical for enamel formation. Mutations of amelogenin cause hypoplastic enamel phenotypes. Previous research found that infant of diabetic mother has higher risk for having enamel hypoplasia. Purpose: The aim of this study was to determine the effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ. Methods: Sixteen female Wistar rats, aged 2.5-3 months, body weight 150-200 g were used in this study, mated then divided into two groups and treated on day 0 of pregnancy. Group A was DM group, consisting of 8 rats, induced by streptozotocin (STZ) injection 40 mg/kg BW. Group B was control group, consisting of 8 rats received citrate buffer injection. Thirty-two rat pups were decapitated on day 5. Immunohistochemical procedures were performed on molar tooth germ of the mandibular rat pups using antibody anti-AMELX to determine the expression of amelogenin. Examination carried out on the images using ImageJ software. All data were then statistically analyzed by Mann Whitney test. Results: There was no significant difference in the expression of amelogenin in the DM group and control group (p>0.05). Conclusion: Gestational diabetes mellitus did not affect the expression of amelogenin in rat offspring tooth germ. Further study is needed to clarify the pattern of amelogenin expression with measurement of glucose levels of rat pups.

Keyword :

Gestational diabetes mellitusn, amelogenin expressio,


References :

Lessi IL,(2010) Evaluation of neonatally-induced mild diabetes in rats: Maternal and fetal repercussions - : Diabetol Metab Syndr





Archive Article

Cover Media Content

Volume : 46 / No. : 3 / Pub. : 2013-09
  1. Correlation Between Salivary Leptin Levels And Incidence Of Caries In Obese Children
  2. The Role Of Calcium On Enamel Hypoplasia Prevention
  3. The Correlation Of Micronucleus Formation And 8-oxo-dg Expression Due To The Panoramic Radiography Exposure
  4. Fluoride Determination Of Children And Adult Toothpaste By Spectrophotometry
  5. Heteroplasmy Analysis Of Dental Pulp Mitochondrial Dna In Forensic Personal Identification
  6. Effect Of Gestational Diabetes Mellitus On The Expression Of Amelogenin In Rat Offspring Tooth Germ
  7. The Effect Of Chitosan Gel Concentration To Neutrophyl And Macrophage In Gingival Ulcer
  8. Epstein-barr Virus (ebv) Dna As Biomaker Of Nasopharyngeal Carcinoma Diagnosis
  9. Metal Crown Restoration With Fiber Post In Young First Permanent Molar
  10. Cranial Size And Cephalic On Index Mentally Retarded 7–12 Years Old Children
  11. Mechanism Of Pomegranate Extract In Killing Malignant Cells In Mice Through Oral Cavity Mucosa Increased Expression Of P53 Wild

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