UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 41 / No. 4 / Published : 2008-10

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Original Article :

Effect of oxygen hyperbaric therapy on malondialdehyde levels in saliva of periodontitis patients with type 2 diabetes mellitus

Author :

  1. Dian Mulawarmanti*1
  2. Widyastuti*2
  1. Department of Biochemistry
    Faculty of Dentistry Hang Tuah University
  2. Department of Periodontology
    Faculty of Dentistry Hang Tuah University

Abstract :

Background: Lipid peroxidation (LPO) has implication in pathogenesis of several pathological disorders including periodontitis. Malondialdehyde (MDA) is end products of upid peroxidation. Hyperbaric Oxygen Therapy (HBOT) involves the administration of 100% oxygen under atmosphere pressure and has been used as an adjuvant therapy, while saliva is a diagnostic tool for many oral and systemic diseases. Purpose: The aim of this study was to examine the effect of HBOT on malondialdehyde in saliva to measure lipid peroxidation in periodontitis patients with type 2 Diabetes Mellitus (DM). Methods: Eight regulated type 2 DM subjects were compared to ten unregulated periodontitis patients type 2 DM (n = 18). Pre HBOT and after 10 days HBOT with 2.4 ATA dose, unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 minutes and were then stored at −80° C until analyzed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. Results: Data showed that regulated type 2 DM had lower level of MDA (3.08 ± 0.62 ug/mol) compared with unregulated periodontally- type 2 DM subjects (5.88 ± 1.04 ug/mol) (p>0.05). MDA levels were significantly lower after HBOT in regulated DM (2.30 ± 0.46 ug/mol) compared with unregulated periodontally type 2 DM (4.09 ± 0.77ug/mol) (p < 0.05). The regulated DM subjects and post HBOT showed MDA levels lower than the periodontally-unregulated group significantly. Conclusion: The saliva of periodontitis patients with unregulated type 2 DM showed more lipid peroxidation than regulated DM type 2. HBOT decreased MDA levels in regulated and unregulated type 2 DM with periodontitis.

Keyword :

hyperbaric oxygen, malondialdehyde, saliva,


References :





Archive Article

Cover Media Content

Volume : 41 / No. : 4 / Pub. : 2009-12
  1. Effect Of Oxygen Hyperbaric Therapy On Malondialdehyde Levels In Saliva Of Periodontitis Patients With Type 2 Diabetes Mellitus
  2. Reducing Allergic Symptoms Through Eliminating Subgingival Plaque
  3. The Role Of Dentists On Medically Compromised Children’s Oral And Dental Prophylaxis In Hospital
  4. The Surface Roughness Difference Between Microhybrid And Polycrystalline Composites After Polishing
  5. The Relation Of Periodontal Diseases To Systemic Diseases
  6. Delayed Bracket Placement In Orthodontic Treatment
  7. Complex Aesthetic Treatment On Anterior Maxillary Teeth With Malposition
  8. Stimulation Of Type I Collagen Activity In Healing Of Pulp Perforation
  9. The Combination Of Sodium Perborate And Water As Intracoronal Teeth Bleaching Agent
  10. Bootstrap Study To Estimate Linear Regression Parameter (application In The Study On The Effect Of Oral Hygiene On Dental Caries)
  11. Immunodetection Of Rasp21 And C-myc Oncogenes In Oral Mucosal Swab Preparation From Clove Cigarette Smokers

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