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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 40 / No. 1 / Published : 2007-01

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Original Article :

Electro-gene therapy in a human oral tongue cancer cell by intratumoral injection of pcdna3._-p27kip_ wt

Author :

  1. Supriatno*1
  1. Department of Oral Medicine, Faculty of Dentistry Gadjah Mada University Yogyakarta - Indonesia

Abstract :

Oral tongue cancers are characterized by a high degree of local invasion and a high rate of metastases to the cervical lymph nodes. Also, treatment options for this cancer are limited. However, a new strategy for refractory cancer, gene therapy is watched with keen interest. Recently, a novel method for high-efficiency and region-controlled in vivo gene transfer was developed by combining in vivo electro-gene therapy and intratumoral plasmid DNA injection. In the present study, a nonviral gene transfer system, in vivo electro-gene therapy in human oral tongue cancer cell, SP-C1 xenograft was examined. The aim of the study is to examine the efficiency of transfection of exogenous p27Kip1 gene by electroporation and the antitumor activity of p27Kip1 gene therapy in human oral tongue cancer xenografts using pcDNA3.1-p27Kip1 wild type (wt) and pcDNA3.1 empty vector with the local application of electric pulses. To evaluate this in vivo gene transfer method, the enhanced green fluorescence protein (EGFP) gene was transfected into xenografts by electroporation. The efficiency of transfection of exogenous p27Kip1 gene by electroporation was confirmed by Western blotting analysis. To estimate the reduction of oral tongue cancer xenografts by this method, the size of SP-C1 xenografts in nude mice after electroporation with wild type p27Kip1 gene was measured. The growth of tumors was markedly suppressed by wild type p27Kip1 gene transfection by electroporation compared with transfection of empty vector only. Moreover, histological specimens revealed apoptotic cell death was increased in wild type p27Kip1-transfected tumors than empty vector. These results suggest that it is possible to transfer wild type p27Kip1 into human oral tongue cancer xenografts using electroporation. Wild type p27Kip1 has a high-potencially to suppress thegrowthoftumors.Finally,combinationsystemofpcDNA3.1-p27Kip1 wt-injectedtumorandelectroporationmightbeusedforhuman oral cancer.

Keyword :

intratumoral, wild type p27Kip1, human oral tongue cancer, electroporation ,


References :

Iga,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma - : International Journal Oncology

Ammar,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma - : International Journal Oncology

Uchida,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma - : International Journal Oncology

Begum,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma - : International Journal Oncology

Tomizuka,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma - : International Journal Oncology

Yoshida,(2004) The clinicopathological significance of the expression of CXCR4 protein in oral squamous cell carcinoma. - : International Journal Oncology

Inagi,(2002) Treatment effects



Archive Article

Cover Media Content

Volume : 40 / No. : 1 / Pub. : 2007-03
  1. Effect Of Combination Antiretroviral Therapy On The Frequency Of Oral Candidiasis In Hiv/aids Patient
  2. Oral And Dental Management In Children With Tetralogy Of Fallot
  3. Understanding About The Classification Of Pulp Inflammation
  4. Electro-gene Therapy In A Human Oral Tongue Cancer Cell By Intratumoral Injection Of Pcdna3._-p27kip_ Wt
  5. Molecular Study Of The Dentin-pulp Complex Responses To Caries Progression
  6. A Comparison Of Three Dimensional Changein Maxillary Complete Dentures Between Conventional Heatpolymerizing And Microwave Polymerizing Techniques
  7. U Bow Activator, An Alternative Functional Orthodontic Appliance
  8. Closure Of Oroantral Fistula With Rotational Palatal Flap Technique
  9. Chronic Periodontitis As An Etiology Of Sleep Disturbances And Premenstrual Syndrome (pms)
  10. The Effect Of Hegu Acupoint Stimulation In Dental Acupuncture Analgesia
  11. Placement Of Fin Type Dental Implant In Three Different Surgical Situations Of Alveolar Bone

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