UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 44 / No. 2 / Published : 2011-06

Order : 3, and page :67 - 71

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Original Article :

Endothelial cell cultured on ha/tcp/chitosan scaffold for bone tissue engineering

Author :

  1. Bachtiar EW*1
  2. Amir LR*2
  3. Utami S*3
  4. Abbas B*4
  1. Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta-Indonesia
  2. Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta-Indonesia
  3. Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta-Indonesia
  4. Center of Education and Isotope Application Technology, BATAN

Abstract :

Background: Angiogenesis is crucial for the success of bone reconstruction through tissue engineering. Currently, is still not known the activity of endothelial cells that is responsible for blood vessel formation, cultured in HA/TCP/chitosan scaffold. The ability of the scaffold to facilitate the proliferation and migration of endothelial cell to form blood vessel is essential for cell survival especially in the inner area of the scaffold that is susceptible for cell death if adequate vascularization is not occurred. Purpose: The purpose of this study was to evaluate the porosity of HA/TCP/chitosan scaffold and the biocompatibility of HA/TCP/chitosan scaffold to endothelial cells. Methods: Endothelial cells were isolated from umbilical vein (human umbilical vein endothelial cells/ HUVEC). HA/ TCP/chitosan scaffold was made from two gelling agents and various basic washing solutions. The characteristic of scaffold was examined by scanning electron microscopy. The activity of HUVEC was evaluated by MTT assay. Results: Initial average scaffold porosity size range from 68 μm and increased up to 134 μm after 7 days incubation with 10 mg/L lysozyme. There was no significant difference in the viability of HUVEC incubated with the scaffold compared to control. Conclusion: HA/TCP/chitosan has a good biocompatibility for HUVEC. This condition supports the activity of HUVEC in the scaffold for angiogenesis process, to provide oxygen and nutrient necessary for osteoblast.

Keyword :

Endothelial cells, scaffold, hydroxyapatite, tri calcium phosphate, chitosan,


References :

Yang S,(2001) he design of scaffolds for use in tissue engineering - : Tissue Eng J





Archive Article

Cover Media Content

Volume : 44 / No. : 2 / Pub. : 2011-06
  1. The Effectivity Of Toothpick Tooth Brushing Method On Plaque Control
  2. Effects Of Different Saliva Ph On Hybrid Composite Resin Surface Roughness
  3. Endothelial Cell Cultured On Ha/tcp/chitosan Scaffold For Bone Tissue Engineering
  4. Elderly Nutritional Status Affect Salivary Anticandidal Capacity Against Candida Albicans
  5. Saliva As A Future Potential Predictor For Various Periodontal Diseases
  6. Relieving Idiopathic Dental Pain Without Drugs
  7. Effectivity Of 0.15% Benzydamine On Radiation-induced Oral Mucositis In Nasopharynx Carcinoma
  8. Constraints On The Performance Of School-based Dental Program In Yogyakarta Province, Indonesia: (a Qualitative Study)
  9. Orthodontic Treatment With Skeletal Anchorage System
  10. The Activity Of Stichopus Hermanii Extract On Triglyceride Serum Level In Periodontitis
  11. Effect Of Soybean Extract After Tooth Extraction On Osteoblast Numbers

Information DENTJ