Dental Journal (Majalah Kedokteran Gigi)
ISSN 1978-3728
Vol. 46 / No. 4 / Published : 2013-12
Order : 4, and page :201 - 205
Related with : Scholar Yahoo! Bing
Original Article :
Fish oil of sardinella longicep regulates osteoblast apoptosis of rat alveolar bone induced lypopolisacharide
Author :
- Didin Erma Indahyani*1
- Department of Oral Biology, Faculty of Dentistry, universitas Jember, Jember - indonesia
Abstract :
Background:Periodontal disease is caused by bacteria periodontopatogen, causing damage to the alveolar bone. The damage due to the imbalance of bone-forming cells, namely osteoblasts and osteoclasts. Decrease in the number of osteoblasts and an increased number of osteoclasts, resulting in bone destruction. The decrease in the number of osteoblasts, due to a disturbance of differentiation, proliferation and apoptosis. Iinflammatory mediators are prostaglandin E2 (PGE2), interleukin-1 (IL-1), IL-6 also tumor necrosis alpha (TNF-α) stimulates osteoblast apoptosis through gene expression, signaling molecules and receptor-forming osteoblasts. Fish oil of lemuru, which is widely encountered in Indonesian coast, containing n-3 poly unsaturated fatty acids (n-3 PUFAs) are quite high. Consumption of fish oil shown to reduce the expression of PGE2, IL-1, IL-6 and TNF-α. Purpose:The purpose of this study was to analyze the effect of lemuru fish oil on osteoblast apoptosis of rat alveolar bone induced periodontal infection. Methods:Thirty Wistar rats, male, age 5 days, divided into 3 groups: group I rats induced with normal saline, group II rats induced by LPS, and group III rats induced with lemuru fish oil and LPS. Each group was divided into 2 sub-groups that will be decapitated at 13 days and 21 days of age. Fish oil given at a dose 1ml/300-350 grams. Lipopolysaccharide (LPS) induced with the purpose to cause periodontal infection in the maxillary buccal fold molar region with dose 5μl LPS/PBS 0.03 ml. After decapitation and decalcification, the maxilla in doing the cutting with a thickness of 5μm. Apoptosis was analyzed on DNA and detected by TUNEL reaction (transferase-mediated digoxigenin-deoxy-UTP nick end labeling). results:The results showed that apoptosis of osteoblast cells was significantly smaller in rats induced by lemuru fish oil. Conclusion:It was concluded that lemuru fish oil containing n-3 PUFAs, namely EPA and DHA, which are crucial to the reduction of osteoblast apoptosis in LPS-induced Wistar rats.
Keyword :
fish oil, osteoblast, apoptosis , n-3 PUFA, periodontal disesase, bone resorption,
References :
Kotake S ,(2010) Effects of NSAIDs on differentiation and function of human and murine osteoclasts– crucial ‘human osteoclastology - : Pharmaceuticals
Archive Article
| Cover Media | Content |
|---|---|
 Volume : 46 / No. : 4 / Pub. : 2013-12 |
|
