Dental Journal (Majalah Kedokteran Gigi)
ISSN 1978-3728
Vol. 47 / No. 4 / Published : 2014-12
Order : 9, and page :215 - 219
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Original Article :
Garlic (allium sativum) extract toxicity test on fibroblast cell culture
Author :
- Yulie Emilda*1
- Els Budipramana*2
- Satiti Kuntari*3
- Mahasiswa Fakultas Kedokteran Gigi
- Dosen Fakultas Kedokteran Gigi
- Dosen Fakultas Kedokteran Gigi
Abstract :
Background: Previous studies have found antimicrobial effect of garlic (allium sativum). Garlic has potential as sterilization material for root canal treatment. Nevertheless, such material has to be non toxic and has to have adequate biocompatibility. Purpose: The study was aimed to examine the toxicity of garlic (allium sativum) on fibroblast cell culture. Method: Toxicity test was conducted using 50%, 75%, 100% of garlic extract, and Chlorphenol Kamfer Menthol (ChKM) as control. BHK-21 cell-culture was put into microplate 96 wells with 2x105 densities and incubated in a 37oC. The garlic extracts in various concentration and ChKM were then placed into the wells. MTT assay test was then use to analyze toxicity, a 50% percentage of living culture-cell was set as a parameter whether the extract is toxic or not. results: The results showed that in 50%, 75%, and 100% garlic concentration indicates a non toxic characteristic on fibroblast cell culture. The non toxic property was consistent in 72, 96, and 120 hours of observation point. Conclusion: The study revealed that garlic on consentration of 50,%, 75%, 100% did not show toxic effect on fibroblast culture cell, but it needs further research for preparing it as an alternative medicament of root canal treatment.
Keyword :
Garlic, allium sativum, toxicity, cell-culture, fibroblast,
References :
Meizarini A ,(2005) Sitoksisitas bahan restorasi cyanoacrylate pada variasi perbandingan powder dan liquid menggunakan esei MTT Surabaya : . Maj. Ked. Gigi (Dent J)
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Volume : 47 / No. : 4 / Pub. : 2014-12 |
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