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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 4 / Published : 2014-12

Order : 9, and page :215 - 219

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Original Article :

Garlic (allium sativum) extract toxicity test on fibroblast cell culture

Author :

  1. Yulie Emilda*1
  2. Els Budipramana*2
  3. Satiti Kuntari*3
  1. Mahasiswa Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: Previous studies have found antimicrobial effect of garlic (allium sativum). Garlic has potential as sterilization material for root canal treatment. Nevertheless, such material has to be non toxic and has to have adequate biocompatibility. Purpose: The study was aimed to examine the toxicity of garlic (allium sativum) on fibroblast cell culture. Method: Toxicity test was conducted using 50%, 75%, 100% of garlic extract, and Chlorphenol Kamfer Menthol (ChKM) as control. BHK-21 cell-culture was put into microplate 96 wells with 2x105 densities and incubated in a 37oC. The garlic extracts in various concentration and ChKM were then placed into the wells. MTT assay test was then use to analyze toxicity, a 50% percentage of living culture-cell was set as a parameter whether the extract is toxic or not. results: The results showed that in 50%, 75%, and 100% garlic concentration indicates a non toxic characteristic on fibroblast cell culture. The non toxic property was consistent in 72, 96, and 120 hours of observation point. Conclusion: The study revealed that garlic on consentration of 50,%, 75%, 100% did not show toxic effect on fibroblast culture cell, but it needs further research for preparing it as an alternative medicament of root canal treatment. 

Keyword :

Garlic, allium sativum, toxicity, cell-culture, fibroblast,


References :

Meizarini A ,(2005) Sitoksisitas bahan restorasi cyanoacrylate pada variasi perbandingan powder dan liquid menggunakan esei MTT Surabaya : . Maj. Ked. Gigi (Dent J)





Archive Article

Cover Media Content

Volume : 47 / No. : 4 / Pub. : 2014-12
  1. The Differences Of Orthodontic Tooth Movement On Menstrual And Ovulation Cycle
  2. The Prevalences Of Early Childhood Caries And Severe Early Childhood Caries In Preschool Children At Gunung Anyar Surabaya
  3. Indirect Pulp Capping In Primary Molar Using Glass-ionomer Cements
  4. Microleakage Of Conventional, Resin-modified, And Nano-ionomer Glass Ionomer Cement As Primary Teeth Filling Material
  5. Initial Acquisition Age Of Mutas Streptococci And Number Of Erupted Primary Teeth In Children
  6. The Antibacterial Effect Of Propolis Additional To Zinc Oxide Eugenol And Zinc Oxide On Polybacteria Of Necrotic Primary Molar
  7. Enamel Micro Hardness Of Young Permanent Tooth After Bleaching And Remineralization Paste Application
  8. Antibacterial Effect Of Mouth Washes Containing Chlorhexidine, Povidone Iodine, Fluoride With Zinc Supplementation On Plaque Polybacteria, Streptococcus Mutans And Porphyromonas Gingivalis
  9. Garlic (allium Sativum) Extract Toxicity Test On Fibroblast Cell Culture
  10. Relationship Between Tweed Triangle And The Position Of Upper Lip And Lower Lip To Esthetic Line