UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 1 / Published : 2012-03

Order : 6, and page :28 - 34

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Original Article :

Genetic variability of candida albicans in hiv/aids patient with and without arv therapy and non hiv/aids

Author :

  1. Retno Puji Rahayu *1
  2. Widiyanti P *2
  3. Arfijanto M *3
  1. Department of Oral Biology Faculty of Dentistry, Airlangga University, Surabaya - Indonesia
  2. Department of Physics, Faculty of Science and Technology, Airlangga University Airlangga University Surabaya - Indonesia
  3. Faculty of Medicine, Airlangga University Surabaya - Indonesia

Abstract :

Background: Oral candidiasis is the mostly found oral manifestation in HIV/AIDS infected patient caused by immunocompromised especially immunodeficiency. Clinical symptoms is severe pain in oral cavity and dry mouth because of xerostomia which cause the loss of appetite. Candida albicans (C. albicans) is normal flora in oral cavity which plays as opportunistic pathogen and also the cause of oral candidiasis. Almost 90% of HIV–infected patient have oral candidiasis. This condition is clinical problem which has not been well-managed yet. C. albicans colonized oral mucous cavity has different genetic variability for each strain. Phenotype of C. albicans has been determined by genetic factor and environtment. This condition stimulate differences of genotype among various strain of C. albicans in the world. Purpose: The purpose of this research is to analyze the genetic variability of C.albicans which colonized in the mucous oral cavity of HIV/AIDS patient in Surabaya in the treatment with and without ARV therapy and non HIV/AIDS. Methods: This research has been identify and characterize the prevalent strain of C. albicans isolat in Surabaya (East Java) in HIV/AIDS infected patient with oral candidiasis by method of Iatron candidal check. The highlight of this research including cytology examination by Papanicoloau staining, C. albicans culture, spheroplast making, DNA isolation and genetic variability checking by randomly amplyfied polymorphism DNA (RAPD). Results: C. albicans colonizing oral mucosa of non-HIV patients had a predisposition of farther genetic relationship (genetic distance of 0.452) with C. albicans colonizing oral mucosa of HIV ARV and HIV non-ARV patients. The genetic distance was ranging between 0 and 1, where 9 was long genetic distance and 1 was short genetic distance. In contrast, C. albicans colonizing oral mucosa of HIV ARV have predisposition of closer genetic relationship (genetic distance of 0.762) with C. albicans colonizing oral mucosa of HIV non-ARV patients. Conclusion: In conclusion, C. albicans which colonized in HIV/AIDS patients in Surabaya with and without ARV therapy have similar genetic variability if compare with non HIV/AIDS patients.

Keyword :

Candida albicans, HIV/AIDS, oral candidosis, RAPD,


References :

Nasronudin.,(2007) Pendekatan biologi molekuler, klinis dan sosial surabaya : Airlangga University Press





Archive Article

Cover Media Content

Volume : 45 / No. : 1 / Pub. : 2012-03
  1. Immediate Overdenture For Improving Aesthetic Of Anterior Teeth With Periodontal Problem
  2. Orthodontic Treatment Considerations In Down Syndrome Patients
  3. The Effective Concentration Of Red Betel Leaf (piper Crocatum) Infusion As Root Canal Irrigant Solution
  4. Degrees Of Chitosan Deacetylated From White Shrimp Shell Waste As Dental Biomaterials
  5. Antimicrobial Effect Of Chlorine Dioxide On Actinobacillus Actinomycetemcomitans In Diabetes Mellitus Rats Treated With Insulin
  6. Genetic Variability Of Candida Albicans In Hiv/aids Patient With And Without Arv Therapy And Non Hiv/aids
  7. Relationship Between Salivary Fluor Concentration And Caries Index In 12–15 Years Old Children
  8. Crude Toxin Of Aggregatibacter Actinomycetemcomitans Serotype-b Increase Parp-1 Expression In Gingival Epithelium
  9. Effectiveness Of Bleaching Agent On Composite Resin Discoloration
  10. Analysis Of Importance Level And Quality Achievement Aspect In Dental Health Service (a Case Study On Waru Sidoarjo Community Dental Health Service)
  11. Effect Of Robusta Coffee Beans Extract Ointment On Full Thickness Wound Healing

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