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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 1 / Published : 2013-03

TOC : 10, and page :45 - 49

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Original Article :

Hemolysin activities as virulence factor of enterococcus faecalis isolated from saliva and periapical abscess (gene detection by pcr)

Author :

  1. Dewa Ayu n.P.a*1
  2. Sari Dewiyani*2
  3. dessy Sulistya ashari*3
  1. Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia
  2. Department of Conservative Dentistry, Faculty of Dentistry, Universitas Prof. Dr. Moestopo
  3. Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia

Abstract :

Background: Enterococcus faecalis is a normal flora of the oral cavity, commonly detected in saliva and persistence in endodontic infections. These bacteria have diverse survival and virulence factors. Hemolysin is one of the factor and still had unclear role as  a virulence factor of the Enterococcus faecalis to survive in the root canal. Purpose: The purpose of this research is to analyze the  presence and activity of hemolysin gene and its activity as a virulence factor isolated from saliva and root canals with periapical  abscess. Yet by understanding one of the phenotypes characters which is hemolysin, it is expected a successful endodontic treatment  can be provided with the persistent of Enterococcus faecalis bacteria. Methods: Method of the research starting with the identification  of Enterococcus faecalis bacteria in isolated saliva and periapical abscess is done in the first part of the study. Then the phenotypes  character of Enterococcus faecalis such as gene detection and expression of hemolysin in blood agar cultures of the 60 colonies samples  performed in the later part. Results: Not all of the colonies cultured were identified as Enterococcus faecalis. All positive detection on  hemolysin gene showed hemolysin expresion in both isolated samples. However, there were samples with hemolysin expression eventough  no hemolysin gene detected. Hemolysin expression detection in saliva was higher due to different activation phase of hemolysin in  saliva. The study with just one primer could lead to the possibility of undetected hemolysin gene, eventough there were samples that  did not have hemolysin gene. The proportion of hemolysin expression in root canals were less than saliva, this could be influenced by  environmental factors. However, Hemolysin was considered as important virulence factor, particularly for disease therapy. Conclusion: The conclusion of this research was hemolysin gene discovered in clinical isolated saliva and root canals samples as virulence factor  of the Enterococcus faecalis, and hemolysin expression occured from both sources.

Keyword :

Hemolysin, virulence, enterococcus faecalis, saliva, root canal,


References :

  1. Portenier I, (2006). nterococcus faecalisthe root canal survival and star in post treatment disease. - : Endodontic Topics


   


Archive Article

Cover Media Content

Volume : 46 / No. : 1 / Pub. : 2013-03
  1. Patient’s expectation on communication performances community of dental health services providers located in urban and rural area
  2. Changes in setting time of alginate impression material with different water temperature
  3. Bactericidal and cytotoxic effects of erythrina fusca leaves aquadest extract
  4. The role of inducible nitric oxide synthase in teeth periapical lesions immunopathogenesis caused by enterococcus faecalis
  5. Antitumor activity of antisense oligonucleotide p45skp2 in soft palate carcinoma cell squamous in vitro
  6. Evaluation of local muscle soreness treatment with anterior bite splint made of soft putty impression material
  7. Unidentified angular recurrent ulceration responsive to antiviral therapy
  8. The inhibition of malignant epithelial cells in mucosal injury in the oral cavity of strains by pomegranate fruit extract (punica granatum linn) through bcl-2 expression
  9. Shear strength of orthodontic bracket bonding with gic bonding agent after the application of cpp-acpf paste
  10. Hemolysin activities as virulence factor of enterococcus faecalis isolated from saliva and periapical abscess (gene detection by pcr)
  11. Effect of citrus aurantifolia swingle essential oils on methyl mercaptan production of porphyromonas gingivalis triggering halitosis