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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 4 / Published : 2013-12

Order : 4, and page :192 - 197

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Original Article :

Inflammatory response of the dental pulp after application of 19% ethylene diamine tetraacetic acid and 37% phosphoric acid as etching agents

Author :

  1. Nadie Fatimatuzzahro*1
  2. Tetiana Haniastuti*2
  3. Juni Handajani*3
  1. Bagian Biomedik Gigi, Fakultas Kedokteran Gigi, Universitas Jember, Jember-Indonesia
  2. Bagian Biologi Oral, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta - Indonesia
  3. Bagian Biologi Oral, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta - Indonesia

Abstract :

Background:Etching agents such as ethylene diamine tetraacetic acid (EDTA) and phosphoric acid which are widely used in adhesive restoration system are aimed to increase retention of restorative materials; however, these agents may induce inflammation of dental pulp. The major function of the inflammatory response is to clear invading pathogens or damaged tissue/ cells and therefore, initiate repair. Neutrophils and macrophages are motile phagocytes that constitute the body’s first line of defense. Purpose:The purpose of the present research was to study the effect of 19% EDTA and 37% phosphoric acid application as an etching agents on the inflammatory response of the dental pulp. Methods:Forty-five male Sprague Dawley rats were divided into 3 groups. Cavity preparation was made on the occlusal surface of maxillary first molar using a round diamond bur. 19% EDTA, 37% phosphoric acid, and distilled water were applied on the surface of the cavity of the teeth in group I, II and III subsequently. The rats were sacrified at 1, 3, 5, 7, and 14 days after the application (n=3 for each day). The specimens were then processed histologically and stained with hematoxylin eosin. results: ANOVA showed a significant difference (p<0.05) among treatment groups, indicating that etching agents application induced neutrophils, macrophages and lymphocytes infiltration in the dental pulp. Tuckey HSD test showed that application of 37% phosphoric acid increased higher number of neutrophils, macrophages and lymphocytes significantly than 19% EDTA (p<0.05). Conclusion:In conclusion, 37% phosphoric acid induced higher number of the inflammatory cells than 19% EDTA.

Keyword :

19% EDTA, 37% phosphoric acid, inflammation, dental pulp,


References :

Roberson TM ,(2006) Sturdevant’s art and science of operative dentistry St. Louis : Mosby Elsevier





Archive Article

Cover Media Content

Volume : 46 / No. : 4 / Pub. : 2013-12
  1. Prevotella Intermedia And Porphyromonas Gingivalis In Dental Caries With Periapical Granuloma
  2. Facial Soft Tissue Profile On Borderline Class I Malocclusion Between Extraction And Non-extraction Orthodontic Treatment
  3. Ekspresi Cox-2 Setelah Pemberian Ekstrak Etanolik Kulit Manggis
  4. Fish Oil Of Sardinella Longicep Regulates Osteoblast Apoptosis Of Rat Alveolar Bone Induced Lypopolisacharide
  5. Inflammatory Response Of The Dental Pulp After Application Of 19% Ethylene Diamine Tetraacetic Acid And 37% Phosphoric Acid As Etching Agents
  6. Potential Of Extracts Cassava Leaf (manihot Utilissima) On The Expression Of Cox-2 On Monocytes That Exposed By Lps E. Coli
  7. The Effect Of Desensitizing Agent Containing Fluoride On Streptococcus Mutans Adhesion And Enamel Roughness
  8. Physical Properties Of Calcite Synthesized Hydroxyapatite As The Filler Of Epoxy-resin-based Root Canal Sealer
  9. Antifungal Property Of Sticophus Hermanii And Holothuria Atra Extract And Its Cytotoxicityto Gingiva-derived Mesenchymal Stem Cell
  10. Orthodontic-surgical Treatment Of A Severe Class Iii Malocclusion
  11. Perbandingan Korelasi Antara Indeks Morfologi Wajah Genap Dengan Sudut Interinsisal Dan Tinggi Wajah Secara Sefalometri

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