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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 4 / Published : 2012-12

Order : 7, and page :212 - 216

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Original Article :

Inhibition effect of cashew stem bark extract (anacardium occidentale l.) on the inhibition of biofilm formation of streptococcus sanguinis

Author :

  1. Rizni Amaliah*1
  2. Sri larnani *2
  3. ivan arie Wahyudi*3
  1. Mahasiswa Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: Biofilm  is  communities  of  microorganisms  attached  to  solid  surface  and  enclosed  in  extracellular  matrix  that protected microorganisms from antibacterial agents and host defense. One of bacteria might have a role in initial colonization of biofilm  formation is Streptococcus sanguinis (S. sanguinis). Previous studies showed that cashew stem bark extract (Anacardium occidentale  L.) can inhibit the growth of Streptococcus strains. Purpose: The purpose of this study was to determine the inhibition effect of cashew  (Anacardium occidentale L.) stem bark ethanol extract on biofilm formation of S. sanguinis. Methods: Streptococcus sanguinis grown  in Brain Heart Infusion (BHI) + 2% sucrose medium by using microplate polystyrene 96 wells. The samples were divided into 3 groups,  5% polyethyleneglycol (PEG) as negative control, cashew stem bark extract (concentration 3.125 mg/ml, 6.25 mg/ml, 9.375 mg/ml,  and 12.5 mg/ml), and 0.12% chlorhexidine (as positive control). Biofilm was stained by 1% crystal violet. Afterwards, optical density  (OD) of samples were measured by microplate reader λ595 nm. The data of biofilm formation inhibition percentage were analyzed by  one way ANOVA and then continued by Least Significant Difference (LSD) test. Results: The result of one way ANOVA showed that  there were significant differences in inhibition of S. sanguinis biofilm formation (p<0.05). LSD test showed that concentration extract  3.125 mg/ml had significant difference with concentration 9.375 mg/ml and 12.5 mg/ml. Reciprocally, concentration 6.25 mg/ml had  significant difference with concentration 9.375 mg/ml and 12.5 mg/ml. Conclusion: Cashew stem bark extract was able to inhibit  biofilm formation of S. sanguinis.

Keyword :

Inhibition of biofilm formation, Streptococcus sanguinis, cashew stem bark,


References :

Socransky SS,(1994) Evidence of bacterial etiology: A historical perspective - : Periodontology 2000





Archive Article

Cover Media Content

Volume : 45 / No. : 4 / Pub. : 2012-12
  1. The Role Of Actinobacillus Actinomycetemcomitans Fimbrial Adhesin On Mmp-8 Activity In Aggressive Periodontitis Pathogenesis
  2. Aesthetic Treatment On Anterior Teeth Crown Fracture Caused By Dental Trauma
  3. Molecular Characterization Of Interleukin-1a (+4845) Gtgene In Aggresive Periodontitis Patients
  4. Antitumor Activity Of Intratumoral Injection Of Pcdna3.1-p27kip1mt Followed By In Vivo Electroporation In A Malignant Burkitt’s Lymphoma Cell Xenograft
  5. The Effect Of Nickel As A Nickel Chromium Restoration Corrosion Product On Gingival Fibroblast Through Analysis Of Bcl-2
  6. Inhibition Of Dental Plaque Formation By Toothpaste Containing Propolis
  7. Inhibition Effect Of Cashew Stem Bark Extract (anacardium Occidentale L.) On The Inhibition Of Biofilm Formation Of Streptococcus Sanguinis
  8. Antibacterial Efficacy Of Salvadora Persicaas A Cleansing Teeth Towards Streptococcus Mutansand Lactobacilli Colonies
  9. Pomegranate Juice As An Ideal Mouthrinse For Fixed Orthodontic Patients
  10. The Increasing Of Fibroblast Growth Factor 2, Osteocalcin, And Osteoblast Due To The Induction Of The Combination Of Aloe Vera And 2% Xenograft Concelous Bovine
  11. The Ph Changes Of Artificial Saliva After Interaction With Oral Ph Changes Of Artificial Saliva After Interaction With Oral Changes Of Artificial Saliva After Interaction With Oral Micropathogen

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