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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 42 / No. 1 / Published : 2009-01

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Original Article :

Inhibitory effect of n-hexane : ethyl acetate fraction from artemisia vulgaris l. on cell culture of epithelial carcinoma of the mouth

Author :

  1. Ira Arundina*1
  1. Department of Oral Biology
    Faculty of Dentistry, Airlangga University
    Surabaya – Indonesia

Abstract :

Background: Sudamala herb (Artemisia vulgaris L.) is often used in society as an anti tumor for organs of digestive system including oral cavity. Nevertheless, there are still no further scientific researches on active materials which can be used as anti carcinogen in oral cavity. Most of anti carcinogens are actually obtained from the genus Artemisia. Moreover, in Indonesia, the species of the genus Artemisia that grows the most is Artemisia vulgaris L. The problem of this research, however, is that the effect of n-hexane fraction: ethyl acetate from Artemisia vulgaris L. towards the decreasing of oncogene in oral squamous cell carcinoma is still indefinite. Purpose: The objective of this research is to explain the effect of giving n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. towards the decreasing of oncogene in oral epithelial carcinoma cell line. Methods: The method of this research is laboratory experimental research by using squamous cell carcinoma of oral cavity on cell culture. The inhibitory percentage test in vitro, furthermore, is taken during the analysis. The result then is analyzed by probit analysis with drawing relation curve between the inhibitory percentage and concentration. Result: The result of n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. has the smallest IC50, 3.902 μg/ml, less than 20 μg/ml suitable with NCI criteria; thus, it can potentially be used as anti carcinogen. Conclusion: There is the decreasing of oncogenes after being given n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. towards oral epithelial carcinoma cell line.

Keyword :

n-hexane: ethyl acetate fraction, Artemisia vulgaris L., oral squamous cells carcinoma,


References :



       


    Archive Article

    Cover Media Content

    Volume : 42 / No. : 1 / Pub. : 2009-03
    1. The Transformation Of Ordinal Scale For Parametric Statistic Analysis On Dental Health Questionnaire
    2. Chronic Gingivitis And Aphthous Stomatitis Relationship Hypothesis: A Neuroimmunobiological Approach
    3. The Role Of Microendodontics In Treating Mandibular Second Molar With Five Canals
    4. Toxicity Testing Of Chitosan From Tiger Prawn Shell Waste On Cell Culture
    5. The Effect Of Toothpaste Containing Kayu Sugi Extract On Plaque Formation
    6. Simple Replantation Protocol To Avoid Ankylosis In Teeth Intended For Orthodontic Treatment
    7. Treatment Of Temporomandibular Dissorder’s Using Occlusal Splint
    8. Inhibitory Effect Of N-hexane : Ethyl Acetate Fraction From Artemisia Vulgaris L. On Cell Culture Of Epithelial Carcinoma Of The Mouth
    9. Antioxidant Effect Of Minocycline In Gingival Epithelium Induced Toxin Actinobacillus Actinomycetemcomitans Serotype B
    10. Esthetic Rehabilitation Of Crowded And Protruded Anterior Dentition
    11. Patient Safety Oriented To Improve Patient Retention In Oral Health Services