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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 2 / Published : 2014-06

Order : 9, and page :103 - 109

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Original Article :

Iron exposure to specific protein expression of extracellular polymeric substance (eps) of aggregatibacter actinomycetemcomitans biofilm

Author :

  1. Marchella Hendrayanti W*1
  2. Indah Listiana Kriswandini*2
  1. Mahasiswa Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: The study of biofilms bacteria could be an alternative of preventive treatment in reducing prevalence of aggressive periodontitis in the community, because biofilm protects the bacteria from environmental conditions, including the attack of immune system and antimicrobial. Aggregatibacter actinomycetemcomitans is a major cause of bacterial aggressive periodontitis. Purpose: This study aims to examine the iron exposure to specific protein expression of extracellular polymeric substance (EPS) of Aggregatibacter actinomycetemcomitans biofilm. Methods: Protein containing EPS biofilm was isolated from cultures of A.actinomycetemcomitans. The protein was processed through several procedures: electrophoresis , electroelution , immunization of rabbits , serum isolation, and purification of antibodies. After the Western blotting procedure the antibody was used. Protein containing EPS biofilms exposed to iron, then once again isolated from cultures of A. actinomycetemcomitans. The electrophoresis and Western blotting were done on the isolated protein. Results: The result showed that the the expression of specific proteins in EPS biofilm decreased in response to iron exposure. Conclusions: Iron exposure could influenced the specific protein expression in EPS biofilm of Aggregatibacter actinomycetemcomitans. 

Keyword :

iron, specific protein, extracellular polymeric substance, biofilm, Aggregatibacter actinomycetemcomitans,


References :

Newman MG ,(2012) Carranza’s clinical periodontology St. Louis : Elsevier Saunders





Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2014-06
  1. Characteristic Evaluation Of Rice Husk Ash With Chitosan High Molecule Nanoparticle As Dentinogenesis Material
  2. The Relationship Determination Between Menarche And The Peak Of Skeletal Maturation Using Hand Wrist And Cervical Vertebrae Inde
  3. The Maturition And Eruption Of Permanent Teeth In Mixed Dentition Children
  4. Surgical Exposure And Orthodontic Treatment On Labially Impacted Maxillary Central Incisor
  5. Reduction Of Salivary Mutans Streptococci In Children With Fixed Orthodontic Appliance After Yoghurt Consumption
  6. Application Of Social Learning Theory In The Management Of Children Dental Fear And Anxiety
  7. Correcting Occlusal Vertical Dimension Using Modified Stainless Steel Crown Restoration In Severe-early-childhood-caries Case
  8. Correction Of Posterior And Anterior Crossbite Using Fixed Orthodontic Appliance With Rapid Maxillary Expansion And Intermaxillary Elastic
  9. Iron Exposure To Specific Protein Expression Of Extracellular Polymeric Substance (eps) Of Aggregatibacter Actinomycetemcomitans Biofilm
  10. Topical Applications Effect Of Casein Phospho Peptide-amorphous Calcium Phosphate And Sodium Fluoride On Salivary Mutans Streptococci In Children
  11. The Characterization Of Stem Cells From Human Exfoliated Deciduous Teeth Using Trypsin Enzym

Information DENTJ