UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 41 / No. 3 / Published : 2008-07

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Original Article :

Isolation and identification of java race amniotic membrane secretory leukocyte protease inhibitor gene

Author :

  1. Elly Munadziroh*1
  1. Department of Dental Material and Technology
    Faculty of Dentistry Airlangga University
    Surabaya - Indonesia

Abstract :

Background: Secretory leukocyte protease inhibitor (SLPI) has been found to facilitate epithelialization, maintain a normal epithelial phenotype, reduce inflammation, secrete growth factors such as IL-4, IL-6, IL-10, EGF, FGF, TGF, HGFand 2-microbulin. SLPI is serine protease inhibitor, which found in secretions such as whole saliva, seminal fluid, cervical mucus, synovial fluid, breast milk, tears, amniotic fluid and amniotic membrane. Impaired healing states are characterized by excessive proteolysis and often-bacterial infection, leading to the hypothesis that SLPI may have a role in the healing process in oral inflammation and contributes to tissue repair in oral mucosa. The oral wound healing response is impaired in the SLPI sufficient mice since matrix synthesis and collagen deposition delayed. The objective of this research is to isolate and identify the amniotic membrane of Java Race SLPI Gene. Methods: SLPI RNA was isolated from Java Race amniotic membrane and the cDNA was amplified by polymerase chain reaction (PCR). Result: Through sequence analyses, SLPI cDNA was 530 nucleotide in length with a predicted molecular mass about 12 kDa. The nucleotide sequence showed that human SLPI from sample was 98% identical with human SLPI from gene bank. PCR analysis revealed that the mRNA of SLPI was highly expressed in the amniotic membrane from Java Race sample. Conclusion: It is demonstrated that human SLPI are highly conserved in sequence content as compared to the human SLPI from gene.

Keyword :

secretory leukocyte protease inhibitor, amniotic membrane, cloning, recombinant, wound healing,


References :





Archive Article

Cover Media Content

Volume : 41 / No. : 3 / Pub. : 2009-09
  1. Cytotoxicity Test Of 40, 50 And 60% Citric Acid As Dentin Conditioner By Using Mtt Assay On Culture Cell Line
  2. Cytotoxicity Of 5% Tamarindus Indica Extract And 3% Hydrogen Peroxide As Root Canal Irrigation
  3. The Effectiveness Of 0.5–0.7% Tetracycline Gel To Reduced Subgingival Plaque Bacteria
  4. The Profile Of Upper Integument Lip Of Baduy And The Nearby Living Sundanese In South Banten, West Java, Indonesia
  5. Isolation And Identification Of Java Race Amniotic Membrane Secretory Leukocyte Protease Inhibitor Gene
  6. The Role Of Proper Treatment Of Maxillary Sinusitis In The Healing Of Persistent Oroantral Fistula
  7. The Management Of Oral Candidosis In Diabetic Patient With Maxillary Herpes Zoster
  8. Non-invasive Endodontic Treatment Of Large Periapical Lesions
  9. Potential Role Of Odontoblasts In The Innate Immune Response Of The Dental Pulp
  10. The Use Of Bay Leaf (eugenia Polyantha Wight) In Dentistry
  11. Effect Of Various Temperature And Storage Duration On Setting Time Of Orega Sealer

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