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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 3 / Published : 2012-09

Order : 5, and page :144 - 149

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Original Article :

Maternal endotoxin-induced fetal growth restriction in rats: fetal responses in toll-like receptor

Author :

  1. Banun Kusumawardani *1
  2. Marsetyawan HNE. Soesatyo*2
  3. Djaswadi Dasuki*3
  4. Widya Asmara*4
  1. Departement of Biomedical, Faculty of Dentistry, Universitas Jember , Jember-Indonesia
  2. Departement of Biomedical, Faculty of Dentistry, Universitas Jember , Jember-Indonesia
  3. Departement of Biomedical, Faculty of Dentistry, Universitas Jember , Jember-Indonesia
  4. Departement of Biomedical, Faculty of Dentistry, Universitas Jember , Jember-Indonesia

Abstract :

Background: Porphyromonas gingivalis as a major etiology of periodontal disease can produce virulence factor, lipopolysaccharide/LPS,which is expected to play a role in the intrauterine fetal growth. Trophoblast at the maternal-fetal interface actively participates in response toinfection through the expression of a family of natural immune receptors, toll-like receptor (TLR). Purpose: the aims of study were to identifyendotoxin concentration in maternal blood serum of Porphyromonas gingivalis-infected pregnant rats, to characterize the TLR-4 expressionin trophoblast cells, and to determine its effect on fetal growth. Methods: Female rats were infected with live-Porphyromonas gingivalis atconcentration of 2 x 109 cells/ml into subgingival sulcus area of the maxillary first molar before and/or during pregnancy. They were sacrifiedon 14th and 20th gestational day. Fetuses were evaluated for weight and length. Endotoxin was detected by limulus amebocyte lysate assayin the maternal blood serum. The TLR-4 expression in trophoblast cells was detected by immunohistochemistry. Results: The mean of LPSconcentrations in maternal blood serum was significantly different (p<0.05) among the four maternal periodontal infection groups. The TLR-4 expressions in syncytiotrophoblast, spongiotrophoblast and trophoblastic giant cells from Porphyromonas gingivalis-infected periodontalmaternal groups were significantly higher than the control group (p<0.05). Maternal endotoxemia affected (p<0.05) the fetal weight andfetal length. Conclusion: The increased LPS concentration in maternal blood serum resulted in the decreased fetal weight and fetal length.Syncytiotrophoblast, spongiotrophoblast and trophoblastic giant cell were able to recognize Porphyromonas gingivalis LPS through theTLR-4 expression. This findings strengthened the link between periodontal disease and fetal growth restriction.

Keyword :

Porphyromonas gingivalis, periodontitis, endotoxin, pregnancy, fetal growth restriction,


References :

Socransky SS,(2005) Periodontal microbial ecology - : Periodontol 2000





Archive Article

Cover Media Content

Volume : 45 / No. : 3 / Pub. : 2012-09
  1. The Benefit Of Differential Moment Concept In Managing Posterior Anchorage And Avoiding Bite Deepening
  2. Henoch-schönlein Purpura In Children: Its Relation To Oral And Dental Health
  3. Distribution Of Class Ii Major Histocompatibility Complex Antigen-expressing Cells In Human Dental Pulp With Carious Lesions
  4. Medication Intake And Its Influence On Salivary Profile Of Geriatric Outpatients In Cipto Mangunkusumo Hospita
  5. Maternal Endotoxin-induced Fetal Growth Restriction In Rats: Fetal Responses In Toll-like Receptor
  6. Novel Development Of Carbonate Apatite-chitosan Scaffolds Based On Lyophilization Technique For Bone Tissue Engineering
  7. Analgesic Effect Of Coconut Shell (cocos Nucifera L) Liquid Smoke On Mice
  8. Penetration Effect Of Prostaglandin E2 Gel On Oral Mucosa Of Rats
  9. Shear Bond Strength Of Self-adhering Flowable Composite On Dentin Surface As A Result Of Scrubbing Pressure And Duration
  10. Facial Reconstruction Using Polypropylene Mesh After Resection Of Maxillary Ossifying Fibroma
  11. Physical Characteristic Of Brown Algae (phaeophyta) From Madura Strait As Irreversible Hydrocolloid Impression Material

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