UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 3 / Published : 2013-09

Related with : Scholar   Yahoo!   Bing

Original Article :

Mechanism of pomegranate extract in killing malignant cells in mice through oral cavity mucosa increased expression of p53 wild

Author :

  1. Sri Hernawati*1
  2. Fedik Abdul Rantam*2
  3. I. Ketut Sudiana*3
  4. A.Retno Pudji Rahayu*4
  1. Bagian Penyakit Mulut, Fakultas Kedokteran Gigi, Universitas Jember, Jember- Indonesia
  2. Departemen Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya - Indonesia
  3. Fakultas Kedokteran, Universitas Airlangga, Surabaya - Indonesia
  4. Departemen Patologi Mulut dan Maksilofasial, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya - Indonesia

Abstract :

Background: Squamous cell carcinoma is a cancer which often occurs in the oral cavity. Microscopic description; squamous epithelial cell proliferation, cell-cell atypia, abnormal keratin formation, irregular cell structure, change the shape of the rete peg processu. DNA examination showed that nearly 90% of cases found the presence of wild p53 gene mutation. wild p53 gene mutation leads to inactivation of p53 wild so it does not stop the cell cycle at G1 phase but it continues. Cancer treatment are still many failures, very expensive, so that requiring treatment alternatives which are cheaper and prospective, such as; the pomegranate (PGL) is one of them. Ellagic acid (EA) of single compounds active ingredients from whole pomegranate fruit extract (PGL) which has activity as in vitro anticancer but  EA  activity and concentrations in the low plasma, low solubility in the water, the metabolism of ellagic acid (EA) is not soluble in the intestinal tract. This fact prompted researcher to compare with  PGL. Purpose: The purpose of this study to compare between  EA  with PGL  in increasing the expression of p53 wild. Methods: The method used is an experimental research laboratories, 32 mice (Balb/c), male, age 5 months were randomly divided into 4 groups: 2 groups of controls (K0: benzopirene not exposed and untreated; K1:  exposed benzopirene and not given treatment); the 2 treatment groups (P1: exposed benzopirene and EA; P2: exposed benzopirene and PGL). Examination using immunohistochemical techniques. Results: The results showed that administration of PGL  can increase the expression of p53 wild- higher than  EA  in malignant epithelial cells in the oral mucosa of mice. Conclusion: Conclusion of this study is the  PGL  can kill malignant cells in the oral cavity of mice by increasing the expression of p53 wild over higher than  EA.  

Keyword :

Pomegranate(PGL), ellagic acid, malignant cells, p53 wild,


References :

Epstein W,(2008) Oral cancer. Texbook of oral medicine Hamilton : BC Decker Inc Press





Archive Article

Cover Media Content

Volume : 46 / No. : 3 / Pub. : 2013-09
  1. Correlation Between Salivary Leptin Levels And Incidence Of Caries In Obese Children
  2. The Role Of Calcium On Enamel Hypoplasia Prevention
  3. The Correlation Of Micronucleus Formation And 8-oxo-dg Expression Due To The Panoramic Radiography Exposure
  4. Fluoride Determination Of Children And Adult Toothpaste By Spectrophotometry
  5. Heteroplasmy Analysis Of Dental Pulp Mitochondrial Dna In Forensic Personal Identification
  6. Effect Of Gestational Diabetes Mellitus On The Expression Of Amelogenin In Rat Offspring Tooth Germ
  7. The Effect Of Chitosan Gel Concentration To Neutrophyl And Macrophage In Gingival Ulcer
  8. Epstein-barr Virus (ebv) Dna As Biomaker Of Nasopharyngeal Carcinoma Diagnosis
  9. Metal Crown Restoration With Fiber Post In Young First Permanent Molar
  10. Cranial Size And Cephalic On Index Mentally Retarded 7–12 Years Old Children
  11. Mechanism Of Pomegranate Extract In Killing Malignant Cells In Mice Through Oral Cavity Mucosa Increased Expression Of P53 Wild

Information DENTJ