UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 45 / No. 3 / Published : 2012-09

Order : 6, and page :150 - 155

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Original Article :

Novel development of carbonate apatite-chitosan scaffolds based on lyophilization technique for bone tissue engineering

Author :

  1. Maretaningtias Dwi Ariani *1
  1. Department of Prosthodontics Faculty of Dentistry, Universitas Airlangga , Surabaya - Indonesia

Abstract :

Background: The natural biopolymer chitosan (Ch) is currently regarded as a candidate for bone tissue engineering. However, Chis poor for cell adhesion and low bone formation ability. In order to enhance cell adhesion and bone formation ability, combination ofCh with carbonate apatite (CA) was developed. Purpose: The aim of this study was to make carbonate apatite-chitosan scaffolds (CAChSs)and evaluate its osteoconductivity in terms of cell proliferation. Methods: Chitosan scaffolds (ChSs) were made by the followingprocedure. Twenty-five, 50, 100, 200 and 400 mg Ch was dissolved into 5 ml of 2% acetic acid (CH3COOH), shaked for 15 min andneutralized with 15 ml of 0.1 M sodium hydroxide (NaOH) solution. After centrifugation, Ch gel was packed into the molds then frozen at-80°C for 2h and dried in a freeze dry machine for 24h. The sponges were subjected to UV radiation for 2h. To make CA-ChSs, 200 mgCh was selected. After neutralization, 50 mg of 0.06 M CA were added into the 200 mg Ch gel. The structure of CA-ChSs was observedby scanning electron microscope (SEM). Mouse osteoblast-like cell (MC3T3-E1) proliferation in these scaffolds was investigated at 1,7, 14 and 21 days. Results: Three dimensional porous structures of CA-ChSs were clearly observed by SEM. Proliferated cell numbersin CA-ChSs was significantly higher than those in ChSs (control) at each stage (p<0.05). Conclusion: It can be concluded that newlydeveloped CA-ChSs had three-dimensional interconnected porous structure, good handling property and supporting ability of proliferationof osteoblasts. It is suggested that newly developed CA-ChSs could be considered as a scaffolds material for bone tissue enginearing.

Keyword :

Carbonate apatite, chitosan, scaffolds, , bone tissue engineering,


References :

Chuna HJ,(2008) Fabrication of porous chitosan scaffold in order to improve biocompatibility - : J Phys Chem of Solids





Archive Article

Cover Media Content

Volume : 45 / No. : 3 / Pub. : 2012-09
  1. The Benefit Of Differential Moment Concept In Managing Posterior Anchorage And Avoiding Bite Deepening
  2. Henoch-schönlein Purpura In Children: Its Relation To Oral And Dental Health
  3. Distribution Of Class Ii Major Histocompatibility Complex Antigen-expressing Cells In Human Dental Pulp With Carious Lesions
  4. Medication Intake And Its Influence On Salivary Profile Of Geriatric Outpatients In Cipto Mangunkusumo Hospita
  5. Maternal Endotoxin-induced Fetal Growth Restriction In Rats: Fetal Responses In Toll-like Receptor
  6. Novel Development Of Carbonate Apatite-chitosan Scaffolds Based On Lyophilization Technique For Bone Tissue Engineering
  7. Analgesic Effect Of Coconut Shell (cocos Nucifera L) Liquid Smoke On Mice
  8. Penetration Effect Of Prostaglandin E2 Gel On Oral Mucosa Of Rats
  9. Shear Bond Strength Of Self-adhering Flowable Composite On Dentin Surface As A Result Of Scrubbing Pressure And Duration
  10. Facial Reconstruction Using Polypropylene Mesh After Resection Of Maxillary Ossifying Fibroma
  11. Physical Characteristic Of Brown Algae (phaeophyta) From Madura Strait As Irreversible Hydrocolloid Impression Material

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