UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 44 / No. 3 / Published : 2011-09

Order : 11, and page :164 - 168

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Original Article :

Odontoblast layer structure alteration as a response to carious lesions

Author :

  1. Tetiana Haniastuti*1
  1. Department of Oral Biology Faculty of Dentistry, Gadjah Mada University Yogyakarta - Indonesia

Abstract :

Background: Dental caries is a bacterial disease affecting the hard tissue of the teeth as well as the pulp. The human dental pulp consists of odontoblast which are organized as a densely packed cell layer. Odontoblasts is located at the periphery of the pulp; therefore, they are the first cells encountered by cariogenic bacteria and their products that are represented in the carious lesion. Purpose: This study aimed to elucidate the effect of cariogenic bacteria to odontoblasts of human teeth. Methods: Five intact third molars and 15 third molars with occlusal caries at various stages of decay were extracted because of orthodontic or therapeutic reasons. The tooth specimens were fixed, decalcified with 10% EDTA solution (pH 7.4), and embedded in paraffin. Serial sections of 5 μm thickness were cut and stained with haematoxylin eosin and Gram’s, in addition to nestin immunohistochemistry. The specimens were then examined under light microscopy. Results: In normal teeth, odontoblast layer were aligned along the pulp chamber showing normal morphology of the cells. Slight disorganization of odontoblast layer was seen in the cases of carious lesions confined to enamel. In the cases of carious lesions confined to dentin, odontoblast layer was not observed in the areas subjacent to the lesions, only single cells showing flattened cell morphology were found. Odontoblasts beneath the lesion suffered severe damage and diminished nestin immunoreaction were observed in all cases of carious lesions with pulp exposure. Conclusion: Cariogenic bacteria invasion may damage the odontoblasts by affecting the morphology and vitality of the cells. The severity of the damage of the odontoblasts may increase as the bacterial invasion progresses toward the pulp.

Keyword :

Dental pulp, odontoblast, carious lesion,


References :

García-Godoy F,(2008) Maintaining the integrity of the enamel surface: The role of dental biofilm, saliva and preventive agents in enamel demineralization and remineralization. - : J Am Dent Assoc





Archive Article

Cover Media Content

Volume : 44 / No. : 3 / Pub. : 2011-09
  1. The Role Of Probiotic On Alveolar Bone Resorption
  2. Dental Measurements Of Deuteromalayid Javanese Students Of The Faculty Of Dentistry Airlangga University
  3. Recent Pharmacological Management Of Oral Bleeding In Hemophilic Patient
  4. Treatment Of Lingual Traumatic Ulcer Accompanied With Fungal Infections
  5. The Effectiveness Of Nigella Sativa Seed Extract In Inhibiting Candida Albicans On Heat Cured Acrylic Resin
  6. Efficacy Of Various Topical Agents To Prevent Enamel Demineralization
  7. Threshold Value Of Enamel Mineral Solubility And Dental Erosion After Consuming Acidic Soft Drinks
  8. Anterior Makeover On Fractured Teeth By Simple Composite Resin Restoration
  9. Management Of Horizontal Crown Fracture Caused By Traumatic Injury With Endorestoration Treatment
  10. Sensitivity Difference Of Streptococcus Viridans On 35% Piper Betle Linn Extract And 10% Povidone Iodine Towards Recurrent Apthous Stomatitis
  11. Odontoblast Layer Structure Alteration As A Response To Carious Lesions

Information DENTJ