UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 44 / No. 4 / Published : 2011-12

Order : 3, and page :177 - 180

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Original Article :

Oral rinse as a potential method to culture candida isolate from aids patients

Author :

  1. Desiana Radithia *1
  2. Hening T. Hendarti*2
  3. Bagus Soebadi*3
  1. Department of Oral Medicine Faculty of Dentistry, Airlangga University Surabaya - Indonesia
  2. Department of Oral Medicine Faculty of Dentistry, Airlangga University Surabaya - Indonesia
  3. Department of Oral Medicine Faculty of Dentistry, Airlangga University Surabaya - Indonesia

Abstract :

Background: Candida isolate is easily sampled from oral cavity by swabbing directly on the candidiasis lesion, to be smeared onto slides for direct examination or cultured in a growth medium. This method is by far the gold standard for defining candidiasis diagnosis. However it is difficult to apply on sensitive patients and almost impossible on patients showing no clinical appearance of oral candidiasis. AIDS patients are very prone to candida infection and have a tendency of repetitive infection involving mixedspecies. As many candida species show different susceptibility to anti-fungal agents, it is necessary to identify the species causing the infection in the management of oral candidiasis. Oral rinse is a suggested method to obtain candida isolate to be cultured for further analysis such as species identification. This method is simple and less risky on infection transmission as less tools are required in the procedure. Purpose: This study aimed to assess the application of oral rinse as an alternative method to culture Candida isolate from AIDS patients. Methods: A cross-sectional observative study was conducted in HIV/AIDS in-Patient Facility of Intermediate Care Unit for Infection Disease, Dr. Soetomo Hospital Surabaya. Fourteen stadium 4 AIDS patients matching criteria were swabbed on 1/3- posterior of the tongue, and then given 10 ml phosphate buffer saline to rinse vigorously for 15 seconds. Both specimens were cultured on Sabouraud’s dextrose agar and colony growth was observed. Results: Candida colonies were able to grow from all 14 isolates (100%) by both methods. Qualitatively, cultures from oral rinse specimens were more populated than cultures from swab specimens. Conclusion: Oral rinse is an applicable technique to obtain Candida species isolate. This technique is safe, easy, non-invasive, and needs less tools therefore less risky for HIV transmission.

Keyword :

HIV/AIDS, oral candidiasis, Candida species, methods for obtaining isolate,


References :

de Andrade AK,(2007) Enamel caries resistance accidentaly irradiated by Nd:YAG Laser. - : Laser Phys Lett





Archive Article

Cover Media Content

Volume : 44 / No. : 4 / Pub. : 2011-12
  1. Betel Leaf Toothpastes Inhibit Dental Plaque Formation On Fixed Orthodontic Patients
  2. In Vivo Characterization Of Polymer Based Dental Cements
  3. Oral Rinse As A Potential Method To Culture Candida Isolate From Aids Patients
  4. In Vitro Effect Of Q-switched Nd:yag Laser Exposure On Morphology, Hydroxyapatite Composition And Microhardness Properties Of Human Dentin
  5. The Effectiveness Of Mimba Oil (azadirachta Indica A. Juss) Spray Disinfectant On Alginate Impression
  6. The Effect Of Spirulina Gel On Fibroblast Cell Number After Wound Healing Process
  7. The Difference Of Residual Monomer Levels Of Acrylic Resin With Various Polymerization Method
  8. Stimulation Of Osteoblast Activity By Induction Of Aloe Vera And Graft Combination
  9. Plaque Index Between Blind And Deaf Children After Dental Health Education
  10. The Difference Of Dental Anxiety Degree In Children Based On The Frequency Of Dental Appointment
  11. Volatile Sulphur Compounds Elimination: A New Insight In Periodontal Treatment
  12. Piperin And Piplartin As Natural Oral Anticancer Drug

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