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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 4 / Published : 2013-12

Order : 5, and page :198 - 203

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Original Article :

Potential of extracts cassava leaf (manihot utilissima) on the expression of cox-2 on monocytes that exposed by lps e. coli

Author :

  1. Zahara Meilawaty*1
  1. Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember Jember - Indonesia

Abstract :

Background:Periodontal disease is a common and widespread disease in the community. Bacteria have a role in periodontitis is a Gram negative bacteria. These bacteria secrete a variety of products such as endotoxin lipopolysaccharide (LPS), which causes the occurrence of inflammation or infection. The first is the body’s defense response of neutrophils and mononuclear cells (monocytes and macrophages). In response to the body’s defenses will be expressed enzyme cyclooxygenase (COX) which functions convert arachidonic acid to prostaglandins. Cassava leaf cells known to play a role in reducing inflammation, but the mechanism for inhibiting COX-2, is not known. Purpose:To analyze of cassava leaf extract on the expression of the enzyme COX-2 in monocytes were exposed to LPS E. coli.Methods: This study is an experimental study in vitro with the design of the posttest only control group design. The independent variable is the extract of the leaves of cassava (Manihot utilissima) at a dose of 12.5 % and 25 %. The dependent variable is the expression of COX-2. COX-2 expression was analyzed by immunocytochemistry method. Isolated monocytes were incubated cassava leaf extract, and then exposed to LPS, after washing imunostaning procedure is then performed using a monoclonal antibody (MAb) anti-human COX-2. The research data is the number of monocytes that express COX-2. results:Expression of COX-2 in the group cassava leaf extract is higher than the group that only LPS E. coli induced. Conclusion: Cassava leaf extract did not inhibit the expression of COX-2. 

Keyword :

LPS, monocytes, COX-2, cassava leaves ,


References :

Greenwall L ,(2001) Bleaching techniques in restorative dentistry London : The Livery House





Archive Article

Cover Media Content

Volume : 46 / No. : 4 / Pub. : 2013-12
  1. Prevotella Intermedia And Porphyromonas Gingivalis In Dental Caries With Periapical Granuloma
  2. Facial Soft Tissue Profile On Borderline Class I Malocclusion Between Extraction And Non-extraction Orthodontic Treatment
  3. Ekspresi Cox-2 Setelah Pemberian Ekstrak Etanolik Kulit Manggis
  4. Fish Oil Of Sardinella Longicep Regulates Osteoblast Apoptosis Of Rat Alveolar Bone Induced Lypopolisacharide
  5. Inflammatory Response Of The Dental Pulp After Application Of 19% Ethylene Diamine Tetraacetic Acid And 37% Phosphoric Acid As Etching Agents
  6. Potential Of Extracts Cassava Leaf (manihot Utilissima) On The Expression Of Cox-2 On Monocytes That Exposed By Lps E. Coli
  7. The Effect Of Desensitizing Agent Containing Fluoride On Streptococcus Mutans Adhesion And Enamel Roughness
  8. Physical Properties Of Calcite Synthesized Hydroxyapatite As The Filler Of Epoxy-resin-based Root Canal Sealer
  9. Antifungal Property Of Sticophus Hermanii And Holothuria Atra Extract And Its Cytotoxicityto Gingiva-derived Mesenchymal Stem Cell
  10. Orthodontic-surgical Treatment Of A Severe Class Iii Malocclusion
  11. Perbandingan Korelasi Antara Indeks Morfologi Wajah Genap Dengan Sudut Interinsisal Dan Tinggi Wajah Secara Sefalometri

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