UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 42 / No. 2 / Published : 2009-04

Related with : Scholar   Yahoo!   Bing

Original Article :

Screening of oral premalignant lesions in smokers using toluidine blue

Author :

  1. Dewi Agustina*1
  2. Yanti Leosari*2
  3. Sri Hadiati*3
  1. Faculty of Dentistry, Gadjah Mada University
    Yogyakarta - Indonesia
  2. Faculty of Dentistry, Gadjah Mada University
    Yogyakarta - Indonesia
  3. Faculty of Dentistry, Gadjah Mada University
    Yogyakarta - Indonesia

Abstract :

Background: A smoker is associated with the risk of developing oral premalignant lesions due to the cacinogenic contents in cigarette. Toluidine blue is a basic chromatic dye used in screening the presence of premalignant lesions due to its ability to detect acidic components in cells and tissues. Purpose: Study was purposed to observe the outcomes of Toluidine blue staining on oral mucosa of smokers and non smokers and to find out whether quantity and duration of smoking affect the final results of Toluidine blue staining. Methods: Forty male subjects, aged 20-60 years old involved in this study, consisted of 10 heavy smokers, 10 moderate smokers, 10 light smokers and 10 non smokers. Subjects were instructed to rinsed their mouths with mineral water for 20 seconds followed by acetic acid 1% for another 20 seconds. Toluidine blue stain was applied in excess and left on site for 1 minute. Subjects were instructed to rinse with acetic acid 1% and sufficient water consecutively for 20 seconds each. The areas of oral mucosa that stained blue were captured with intraoral camera and transferred to the computer unit. The staining procedure was repeated after 14 days. Results: Chi-square test showed that Toluidine blue positive staining dominates the smokers group. Regression and correlation test indicate that Toluidine blue staining is more obvious in subjects who consume more cigarettes. Conclusion: It was concluded that oral mucosa of smokers absorbed more Toluidine blue than that of non smokers and retention of Toluidine blue is affected by quantity and duration of smoking.

Keyword :

cigarette, oral mucosa, Toluidine blue, smokers,


References :





Archive Article

Cover Media Content

Volume : 42 / No. : 2 / Pub. : 2009-06
  1. Diagnosis And Management Of Crohn’s Disease In Retarded Child
  2. Pseudomembranous Candidiasis In Patient Wearing Full Denture
  3. Cooperation Of Patient As Key Factor To Overcome Oral Habits
  4. Transformation Analysis Of Oral Epithelial Dysplasia To Cis And Scc By P53 Expression And Gene Mutations
  5. Aesthethic And Masticatory Rehabilitation On Post Mandibular Resection With Combination Of Hollow Obturator And Hybrid Prosthesis
  6. The Effect Of Watermelon Frost On Prostaglandin E2 (pge2) In Inflamed Pulp Tissue (in Vitro Study)
  7. Shear Bond Strength Between Porcelain And Nano Filler Composite Resin With Or Without 9% Hydrofluoric Acid Etching
  8. Screening Of Oral Premalignant Lesions In Smokers Using Toluidine Blue
  9. Biocompatibility Of Acrylic Resin On Bhk–21 Cell Line After Being Soaked In Sodium Hypochlorite
  10. The Aesthetic Treatment For Anterior Teeth With Lost Crown, By Endorestoration
  11. Prognostic Value Of Molecular Markers Of Oral Pre-malignant And Malignant Lesions

Information DENTJ