UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 39 / No. 1 / Published : 2006-01

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Original Article :

Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits

Author :

  1. Freeda Woon Faiza Woon Tai Keat*1
  2. Kannan*2
  3. Widowati Witjaksono*3
  4. Abdul Rani Samsudin*4
  1. School of Dental Sciences, University Sains Malaysia Kubang Kerian, Kelantan - Malaysia
  2. School of Dental Sciences, University Sains Malaysia Kubang Kerian, Kelantan - Malaysia
  3. School of Dental Sciences, University Sains Malaysia Kubang Kerian, Kelantan - Malaysia
  4. School of Dental Sciences, University Sains Malaysia Kubang Kerian, Kelantan - Malaysia

Abstract :

Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine the cytotoxicity in terms of mitotic index as well as the clastogenic effect (chromosome aberration) of sea coral implantation in rabbits. The animals comprised of five male adult healthy New Zealand White (Oryctolagus cuniculus) rabbits. The biomaterial, sea coral granules used in this study was obtained from Porites species and processed by the tissue bank of Universiti Sains Malaysia, Health Campus, Kubang Kerian, Malaysia. The blood samples were collected twice from the rabbits, once before the implantation of the sea coral granules (which acted as the control) and the other, one week after the implantation (which acted as the treatment) and lymphocyte cultures were set up. The cultures were then harvested and the chromosomes were prepared for analysis. The diploid number of chromosomes in the rabbits (Oryctolagus cuniculus) was found to be 44. Mean mitotic indices of 3.84 ± 0.54 per cent and 3.76 ± 0.23 per cent were obtained before and after implantation of sea coral granules respectively. There were no structural or numerical chromosomal aberrations observed in both the cases. The mitotic index values and chromosomal analyses in this preliminary study carried out indicate that the biomaterial, sea coral granules is non-cytotoxic and non-clastogenic under the present test conditions.

Keyword :

sea coral, cytotoxicity, chromosome aberration ,


References :

Tuominen,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Orthopaedics

Jämsä,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Orthopaedics

Tuukkanen,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Orthopaedics

Nieminen,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Orthopaedics

Lindholm,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Orthopaedics

Lindholm,(2000) Native bovine bone morphogenetic protein improves the potential of biocoral to heal segmental canine ulnar defects - : International Ort



Archive Article

Cover Media Content

Volume : 39 / No. : 1 / Pub. : 2006-03
  1. Effects Of Irrigation Solutions And Calcium Hydroxide Dressing On Root Canal Treatments Of Periapical Lesions
  2. Lip And Allar Base Dimensions Among Adolescence In Healthy And Post Unilateral Cleft Lips Correction In Deutro-malaid Population
  3. Study On The Cytotoxicity And Chromosome Aberration Following Implantation Of Sea Coral In Rabbits
  4. Antimicrobial Effects Of Coleus Amboinicus, Lour Folium Infusum Towards Candida Albicans And Streptococcus Mutans
  5. Manufacturing Hollow Obturator With Resilient Denture Liner On Post Hemimaxillectomy
  6. Poor Oral Hygiene As Trigger Of Diabetes Mellitus Progressiveness
  7. Inhibition Effect Of Calcium Hydroxide Point And Chlorhexidine Point On Root Canal Bacteria Of Necrosis Teeth
  8. Hybrid Layer Difference Between Sixth And Seventh Generation Bonding Agent
  9. Dental Root Periapical Resorption Caused By Orthodontic Treatment
  10. The Effect Of Caffeine On Osteoblast Proliferation After Tooth Extraction In Wistar Rats

Information DENTJ