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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 1 / Published : 2014-03

Order : 8, and page :41 - 44

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Original Article :

The role of heat shock protein 27 (hsp 27) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture

Author :

  1. Sri Wigati Mardi Mulyani*1
  2. Ernie Maduratna Setiawati*2
  3. Erma Safitri*3
  4. Eha Renwi Astuti*4
  1. Dosen Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption  in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in  the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition.  Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the  stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of casase 9. Purpose:  The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow  stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis  through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR.  Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of Caspase 9. Conclusion: Hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9. 

Keyword :

Mesenchymal stem cells, hypoxia, HSP 27, caspase-9, apoptosis,


References :

Coppess RP ,(2011) Stem cells and the repair of radiationinduced salivary gland damage - : J Oral Disease





Archive Article

Cover Media Content

Volume : 47 / No. : 1 / Pub. : 2014-03
  1. Combination Of Aloe Vera And Xenograft Induction On Decreasing Of Nf-kb Of Tooth Extraction Socket Preservation In Cavia Cobaya
  2. Role Of Hsp60, Cd-8 And Ifn-γ On The Imunopatobiogenesis Of Periapical Granuloma On Dental Caries
  3. The Expressions Of Nf-κβ And Tgfβ-1 On Odontoblast-like Cells Of Human Dental Pulp Injected With Propolis Extracts
  4. Induction Of Spirulina Chitosan Gel Into Cavia Cobaya Socket Toward Healing Process Post Extraction
  5. Alkaline Phosphatase Expression During Relapse After Orthodontic Tooth Movement
  6. Antimicrobial Proteins Of Snail Mucus (achatina Fulica) Against Streptococcus Mutans And Aggregatibacter Actinomycetemcomitans
  7. The Effect Of Immersion In Soda On Nano Hybrid Composite Resin Discoloration
  8. The Role Of Heat Shock Protein 27 (hsp 27) As Inhibitor Apoptosis In Hypoxic Conditions Of Bone Marrow Stem Cell Culture
  9. The Sensitivity And Specificity Test Of Pediatric Caries Predictor Software
  10. The Effect Of Position And Volumetric Fraction Polyethylene Fiber On The Flexural Strength Of Fiber Reinforced Composite
  11. Oral Hygiene And Number Of Oral Mucosal Lesion Correlate With Oral Health-related Quality Of Life In Elderly Communities Of Yogyakarta City

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