UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 40 / No. 4 / Published : 2007-10

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Original Article :

The citotoxicity of calcium hydroxide intracanal dressing by mtt assay

Author :

  1. Nanik Zubaidah*1
  1. Department of Conservative Dentistry, Faculty of Dentistry Airlangga University, Surabaya - Indonesia

Abstract :

Calcium hydroxide had been used as the intracanal dressing in endodontic treatment due to its high alkaline and high antimicrobial capacity. It also be able to dissolve the necrotic tissue, prevent the root resorbtion and regenerate a new hard tissue. The aim of this study is to identify the concentration of calcium hydroxide that has the lowest citotoxicity. There are 5 groups, each group had 8 samples with different concentration of calcium hydroxide. Group II: 55%, Group III: 60%, Group IV: 65% and Group V: 70%. The citotoxicity test by using enzymatic assay of MTT [3-(4.5-dimethylthiazol-2yl) ]-2.5 diphenyl tetrazolium bromide, against fibroblast cell (BHK-21). The result of susceptibility test was showed by the citotoxicity detection of the survive cell of fibroblast that was measured spectrophotometrically using 595 nm beam. The data was analyzed using One-Way ANOVA test with significant difference 0.05 and subsequently LSD test. The result showed that in concentration 50%, 55%, 60%, 65%, and 70% calcium hydroxide had low toxicity, but calcium hydroxide 60%, had the lowest toxicity.

Keyword :

calcium hydroxide, citotoxicity, MTT assay ,


References :





Archive Article

Cover Media Content

Volume : 40 / No. : 4 / Pub. : 2007-12
  1. The Management Of Oral Erythema Multiforme In Juvenile Patient
  2. The Citotoxicity Of Calcium Hydroxide Intracanal Dressing By Mtt Assay
  3. The Correction Of Occlusal Vertical Dimension On Tooth Wear
  4. The Effect Of Gender Differences On Dentist's Performance (a Study In Health Centers In East Java)
  5. Various Polymerization Temperature On Dimensional Accuracy Of Orthodontic Acrylic Base Plate
  6. The Ability Of 5% Tamarindus Indica Extract As Cleaner Of The Root Canal Wall Smear Layer
  7. A New Concept In Orthodontics: Faster And Healthier Tooth Movement By Regularly Consuming Xyilitol Chewing Gum
  8. The Application Of Methacrylate Resin And The Derivation As Restorative Material Of Damaged Tooth Tissue
  9. The Clinical Potential And Limits Of The All-ceramic Fixed Partial Denture Restorations
  10. Closed Mouth Method With Dynamic And Muco Compressive Impression On Upper And Lower Jaw Ridges For Aid Full Denture Retention

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