Catalog :




Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 46 / No. 3 / Published : 2013-09

Related with : Scholar   Yahoo!   Bing

Original Article :

The correlation of micronucleus formation and 8-oxo-dg expression due to the panoramic radiography exposure

Author :

  1. Rurie Ratna Shantiningsih*1
  2. Suwaldi*2
  3. Indwiani Astut*3
  4. Munakhir Mudjosemedi*4
  1. Department of Dentomaxillofacial, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta – Indonesia
  2. Faculty of Pharmacy; Universitas Gadjah Mada, Yogykarta – Indonesia
  3. Departement Pharmacology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta – Indonesia
  4. Department of Dentomaxillofacial, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta – Indonesia

Abstract :

Background: The expression of 8-oxo-dG is defined as one form of damaged DNA occuring as the result of oxidation reaction due to X ray exposure. Panoramic radiography exposure has been widely known to be able to increase micronucleus which are signing the early stage and as biomarker in carsinogenesis mechanism. Purpose:The purpose of this research was to study the correlation between micronucleus number and 8-oxo-dG expression as a result of panoramic radiography exposure. Methods:Twelve New Zealand male rabbits aging 6 months were divided into 4 groups. Group I were rabbits that represented 0 day, group II represented 3th day, group III represented 6th day, and group IV represented 9th day after the panoramic radiography exposure. Respectively samples were swabbed at mandibular anterior gingival mucosa before and after the panoramic radiography exposure. The swabbed samples were coloured by using Feulgen-Rossenbeck staining for calculating the amount of micronucleus formation. Expression of 8-oxo-dG was detected using immunohistochemical of rabbit’s gingival mucosa epithelium. Statistical analysis were carried out on pearson correlation. Results: There was a highest increasing of micronucleus on the 9th day after panoramic radiography exposure. Meanwhile, there was significant correlation (p=0,049) of the increasing amount of micronucleus and 8-oxo-dG expression in negatively correlation (r = -0,578). The increasing of micronucleus formation on the 9th day after panoramic radiography exposure was accordance with some previous studies. The expression score of 8-oxo-dG decreases as the day goes by. Conclusion:There was a correlation between the number of micronucleus and expression score of 8-oxo-dG.

Keyword :

Micronucleus, 8-oxo-dG, radiography panoramic exposure, rabbit,


References :

  1. Whaites E, (2002). Essentials of dental radiography and radiology . London : Churchill Livingstone


   


Archive Article

Cover Media Content

Volume : 46 / No. : 3 / Pub. : 2013-09
  1. Correlation between salivary leptin levels and incidence of caries in obese children
  2. The role of calcium on enamel hypoplasia prevention
  3. The correlation of micronucleus formation and 8-oxo-dg expression due to the panoramic radiography exposure
  4. Fluoride determination of children and adult toothpaste by spectrophotometry
  5. Heteroplasmy analysis of dental pulp mitochondrial dna in forensic personal identification
  6. Effect of gestational diabetes mellitus on the expression of amelogenin in rat offspring tooth germ
  7. The effect of chitosan gel concentration to neutrophyl and macrophage in gingival ulcer
  8. Epstein-barr virus (ebv) dna as biomaker of nasopharyngeal carcinoma diagnosis
  9. Metal crown restoration with fiber post in young first permanent molar
  10. Cranial size and cephalic on index mentally retarded 7–12 years old children
  11. Mechanism of pomegranate extract in killing malignant cells in mice through oral cavity mucosa increased expression of p53 wild