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Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 3 / Published : 2014-09

Order : 4, and page :135 - 140

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Original Article :

The effect of aspirin administration length on ki-67 expression protein and oral epithelial mucosal thickness in male wistar mice

Author :

  1. Dian Yosi Arinawati*1
  2. Heni Susilowati*2
  3. Supriatno*3
  1. Departemen Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta
  2. Departemen Biologi Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta
  3. Departemen Ilmu Penyakit Mulut, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta

Abstract :

Background: Aspirin has been widely used as an anti-inflammatory, antipyretic, and analgesics drugs. It has seriously side effects like gastrointestinal ulceration, delayed healing ulcer, and oral mucosal ulceration when Aspirin is administered for long time. Purpose: The aim of this study was to examine the effect of Aspirin administration on Kiehl-67 (KI-67) protein expressions and oral mucosal epithelial thickness in male Wistar mice. Methods: Experimental laboratory study with post-test only control group design was performed and 40 male Wistar mice were used in this experiment. The samples were divided into 2 groups. Group I was treated with Aspirin, whereas group II was receive aquadest. Each group was divided into 5 subgroups for assessment of the length administration effect. All of mice were sacrificed on day 1, 3, 5, 7 and 10 after treatment. Aspirin was orally administrated with doses of 9 mg/kg body weight. The buccal right of mice oral mucosal tissue was sliced and delivered for immunohistochemistry staining using anti-KI-67. Hematoxylin-eosin (HE) staining was performed to measure the oral epithelial thickness. Examination of KI-67 expressions and oral epithelial thickness were performed by using ImageJ software. Two-way Anova and Kruskall-Wallis test were carried-out for data analysis with significant level of 95%. Results: The results revealed that the administration of Aspirin in mice on day 1, 3, 5, 7, and 10 was markedly decreased in the KI-67 protein expressions and oral epithelial thickness compared with that of control (p<0.05), otherwise the duration of Aspirin administration did not affect mucosal epithelial thickness. Conclusion: Aspirin administration length has the potential to suppress the KI-67 protein expression within 10 days; the effect in line with the length of duration. The epithelial thickness was not influenced by the length of Aspirin administration. 

Keyword :

Aspirin, KI-67 expression, oral mucosal epithelial thickness, Wistar mice,


References :

Shah JS ,(2012) Short communication: anti-ulcer activity of lucer against experimentally induced gastric ulcers in rats - : Ayu J





Archive Article

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Volume : 47 / No. : 3 / Pub. : 2014-09
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  2. Modulation Of Fgf2after Topical Application Ofr Topical Application Of Topicalapplication Ofapplication Of�tichopus Hermaniigelontichopus Hermaniigelon Traumatic Ulcer In Wistar Rats
  3. Comparison Of Three Types Algyrogel Cytotoxicity Against Fibroblasts
  4. The Effect Of Aspirin Administration Length On Ki-67 Expression Protein And Oral Epithelial Mucosal Thickness In Male Wistar Mice
  5. Characterization Of Lactoferrin In Gingival Crevicular Fluid Of Chronic Periodontitis Patient
  6. Oral Health Knowledge Among Parents Of Autistic Child In Bandung-indonesia
  7. Effect Of Siwak (salvadora Persica) Traditional Toothbrush On The Accumultated Dental Plaque
  8. The Management Of Impacted Permanent Canine With Surgical Exposure
  9. Inhibition Effect Of Xylitol And Nistatin Combination On Candida Albicans Growth (in Vitro)
  10. The Release Of Nickel And Chromium Ions In Special Type Of Australian Wire And Stainless Steel In Artificial Saliva
  11. Identifikasi Bakteri Saat Insial Akuisisi Streptococcus Mutans Pada Bayi

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