UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 41 / No. 2 / Published : 2008-04

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Original Article :

The effect of exposure duration of self etch dentin bonding on the toxicity of human gingival fibroblast of cell culture

Author :

  1. Sri Lestari*1
  1. Department of Conservative Dentistry
    Faculty of Dentistry Jember University
    Jember - Indonesia

Abstract :

Self etch dentin bonding created to make light easily activate the application of composite resin on tooth surface. The monomer content has acid effect that could irritate tooth pulp. The purpose of this study was to evaluate the effect of light exposure duration of self etch dentin bonding on toxicity of human gingival fibroblast of cell culture by MTT assay. Self etch dentin bonding was used as on experimental unit and the sample was exposed by visible light curing in different duration: 10, 20, 30 seconds and immerged in artificial saliva in pH 7 for 24 hours. 100 µl artificial saliva was exposed to human gingival fibroblast of cell culture 20.000 cells/100 µl RPMI for 24 hours. Toxicity was evaluated by MTT assay, optical density was measured using 550 nm spectrophotometer. The data was analyzed using Kruskal Wallis in 5% degree of significance. The result showed that increasing exposure duration (10, 20, 30 seconds) of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture. It is concluded that 30 seconds-exposure of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture.

Keyword :

exposure duration, self etch dentin bonding, toxicity, MTT assay ,


References :





Archive Article

Cover Media Content

Volume : 41 / No. : 2 / Pub. : 2008-06
  1. The Role Of Partial Denture In Management Of Hypohidrotic Ectodermal Dysplasia
  2. Management Of Zygomatic-maxillary Fracture (the Principles Of Diagnosis And Surgical Treatment With A Case Illustration)
  3. The Transversal Strength Of Acrylic Resin Plate After Soaking In The Juice Of The Noni Fruit (morinda Citrifolia Linn.)
  4. Pulp Tissue Inflammation And Angiogenesis After Pulp Capping With Transforming Growth Factor β1
  5. The Effect Of Exposure Duration Of Self Etch Dentin Bonding On The Toxicity Of Human Gingival Fibroblast Of Cell Culture
  6. Effect Of Il-1 And Gustducin Expression Change On Bitter Taste During Fever
  7. Differences In Cytotoxicity Between 5% Tetracycline Hydrochloride And 15% Edta As Root Canal Irrigant
  8. The Level Of Streptococcus Mutans And Lactobacillus In Saliva Of Dental Caries And Free Caries Children
  9. Combination Of Natural Teeth And Osseointegrated Implants As Prosthesis Abutments In A Posterior Cantilever Bridge
  10. Students’ Evaluation Of Preclinical Simulation For All Ceramic Preparation (in Faculty Of Dentistry Universiti Kebangsaan Malaysia)
  11. Potency Of Probiotic Therapy For Dental Caries Prevention
  12. The Use Of 90% Aloe Vera Freeze Drying As The Modulator Of Collagen Density In Extraction Socket Of Incicivus Cavia Cobaya

Information DENTJ