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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 47 / No. 1 / Published : 2014-03

TOC : 3, and page :13 - 18

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Original Article :

The expressions of nf-κβ and tgfβ-1 on odontoblast-like cells of human dental pulp injected with propolis extracts

Author :

  1. Ira Widjiastuti*1
  2. Ketut Suardita *2
  3. Widya Saraswati*3
  1. Dosen Fakultas Kedokteran Gigi
  2. Dosen Fakultas Kedokteran Gigi
  3. Dosen Fakultas Kedokteran Gigi

Abstract :

Background: Propolis is known to have beneficial effects, namely anti- bacterial, anti-viral, anti-inflammatory, antioxidant, and immunomodulatory. Propolis extracts with anti-inflammatory properties are expected to be useful in treating inflamed pulp tissue with a diagnosis of reversible pulpitis. The inflammation of pulp tissue is caused by bacteria, namely Lactobacillus acidophilus. This research used odontoblast like cells derived from pulp tissue of human third molars. Odontoblast like cells exposed to Lactobacillus achidophilus were used as a model of proinflammatory cytokine signaling. This research examined the effects of propolis extracts on odontoblast like cells exposed to Lactobacillus acidophilus. Purpose: This research aimed to determine the effectiveness of propolis extracts on the activities of odontoblast-like cells exposed to Lactobacillus acidophillus by measuring the expressions of NF-κβ and TGF- β1. Methods: First, pulp odontoblast cultures were derived from human dental pulp tissues of impacted third molars removed by using digestion method. Next, odontoblast-like cells exposed to inactive Lactobacillus acidophilus bacteria were given propolis extract. Finally, the activities of odontoblast-like cells were monitored by measuring the expressions of NF-kβ and TGFβ-1 with immunocytochemistry technique. Results: A decline NF-kβ expression and on increase of TGFβ-1 expression on odontoblast like cells exposed to inactive Lactobacillus acidophilus. Conclusion: Propolis extracts inhibit the expression of NF-kβ, and increase the expression of TGF-β1 in pulp odontoblast-like cells exposed to inactive Lactobacillus acidophillus.    

Keyword :

Propolis extracts, odontoblast-like cells, Lactobacillus acidophillus, NF-kβ, TGFβ-1,


References :

  1. Smith AJ , (2003). Vitality of the dentin-pulp complex in health and disease: growth factors as key mediators. - : J Dent Educ


   


Archive Article

Cover Media Content

Volume : 47 / No. : 1 / Pub. : 2014-03
  1. Combination of aloe vera and xenograft induction on decreasing of nf-kb of tooth extraction socket preservation in cavia cobaya
  2. Role of hsp60, cd-8 and ifn-γ on the imunopatobiogenesis of periapical granuloma on dental caries
  3. The expressions of nf-κβ and tgfβ-1 on odontoblast-like cells of human dental pulp injected with propolis extracts
  4. Induction of spirulina chitosan gel into cavia cobaya socket toward healing process post extraction
  5. Alkaline phosphatase expression during relapse after orthodontic tooth movement
  6. Antimicrobial proteins of snail mucus (achatina fulica) against streptococcus mutans and aggregatibacter actinomycetemcomitans
  7. The effect of immersion in soda on nano hybrid composite resin discoloration
  8. The role of heat shock protein 27 (hsp 27) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture
  9. The sensitivity and specificity test of pediatric caries predictor software
  10. The effect of position and volumetric fraction polyethylene fiber on the flexural strength of fiber reinforced composite
  11. Oral hygiene and number of oral mucosal lesion correlate with oral health-related quality of life in elderly communities of yogyakarta city