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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 43 / No. 4 / Published : 2010-12

TOC : 2, and page :168 - 171

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Original Article :

The increasing of odontoblast-like cell number on direct pulp capping of rattus norvegicus using chitosan

Author :

  1. Widyasri Prananingrum*1
  1. Department of Material Science and Technology Faculty of Dentistry, Hang Tuah University Surabaya - Indonesia

Abstract :

Background: Pulpal perforation care with direct pulp capping in the case of reversible pulpitis due to mechanical trauma was performed with chitosan which has the ability to facilitate migration, proliferation, and progenitor cell differentiation. Purpose: The purpose of this study was to determine the increasing number of odontoblast-like cells in direct pulp capping dental care of Rattus norvegicus using chitosan for seven and fourteen days. Methods: Samples were molars of male Rattus norvegicus strain wistar, aged between 8–16 weeks, divided into two treatment groups, namely group I given chitosan and group II as a control group given Ca(OH)2. Those Rattus norvegicus’ occlusal molar teeth were prepared with class I cavity, and then chitosan and Ca(OH)2 were applied as the pulp capping materials. Afterwards, glasss ionomer cement type IX was used as a restoration material. Their teeth and jaw were then cut on the seventh day and the fourteenth day. Next, histopathological examination was carried out to observe the odontoblast like cells. All data were then analyzed by t test. Degree of confidence obtained, finally, was 95%. Results: The results obtained showed that the significant differences of odontoblast like cells on the seventh day observation was 0.001 (p=0.001), and on the fourteenth day observation was 0.002 (p=0.002). Conclusion: The number of odontoblast-like cells in direct pulp capping dental care of rattus norvegicus using chitosan is higher than the one using Ca(OH) for seven and fourteen days.

Keyword :

Chitosan, calcium hydroxide, direct pulp capping, odontoblast-like cells,


References :

  1. Maretaningtias DA, (2009). Toxicity testing of chitosan from tiger prawn shell waste on cell culture. Surabaya : Dent J (Maj. Ked. Gigi)


   


Archive Article

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Volume : 43 / No. : 4 / Pub. : 2010-12
  1. Clinical consideration of thrombocytopenia in children
  2. The increasing of odontoblast-like cell number on direct pulp capping of rattus norvegicus using chitosan
  3. Minor modification of millard surgical technique for correction of complete unilateral cleft lip
  4. Biocompatibility and osteoconductivity of injectable bone xenograft, hydroxyapatite and hydroxyapatite-chitosan on osteoblast culture
  5. Alveolar ridge rehabilitation to increase full denture retention and stability
  6. Pulp nerve fibers distribution of human carious teeth: an immunohistochemical study
  7. The management of dental fracture on tooth 61 in a child with attention deficit hyperactivity disorders
  8. Multidisciplinary management of a mandibular buccal plate perforation
  9. Candida albicans adherence on acrylic resin plates immersed in black tea steeping
  10. A combination of endodontic therapy and root resection in furcation involvement case
  11. interleukin-1β expression among periodontitis patients in surabaya