Dental Journal (Majalah Kedokteran Gigi)
ISSN 1978-3728
Vol. 46 / No. 1 / Published : 2013-03
Order : 8, and page :35 - 38
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Original Article :
The inhibition of malignant epithelial cells in mucosal injury in the oral cavity of strains by pomegranate fruit extract (punica granatum linn) through bcl-2 expression
Author :
- Sri Hernawati *1
- Department of Oral Medicine, Faculty of Dentistry, Universitas Jember, Jember- Indonesia
Abstract :
Background: Squamous cell carcinoma of oral cavity is a malignant neoplasms derived from epithelia. The malignant neoplasms are cells that have changed their structure and function, and their number becomes increasing abnormally, invasive, and metastatic. Carcinoma can be caused by the resistance of malignant cell apoptosis. Bcl-2 is a proto-oncogene of Bcl family that inhibits the process of cell apoptosis and suppresses Bax protein (pro-apoptotic). The management efforts of cancer diseases, however, still have many obstacles. Thus, the researcher was triggered to explore more herbal plants, namely pomegranate. Pomegranate as a medicinal plant is accessible and cheap. Ellagic acid (EA) is a single active compound derived from whole pomegranate fruit extract (PGL), which has anti-cancer activity as in vitro, but EA is low concentration in plasma, low water solubility, and insoluble in intestinal. These facts prompted the researcher to compare between pomegranate extract, which consists of several active compounds, and that, which only consists of ellagic acid. Thus, this research is expected to know how some active compounds can work synergistically in the PGL, so the effect can be more potent. Purpose: The purpose of this research, therefore, was to compare EA with PGL in reducing the expression of Bcl-2. Methods: This laboratory experimental research was used 32 male mice (Balb/c) in the age of 5 months. They were randomly divided into 4 groups: 2 control groups (K0), which was not exposed with benzopirene and also untreated; and K1, which was exposed with benzopirene and also untreated), 2 treatment groups (P1, which was exposed with benzopirene and also treated with EA; and P2, which was exposed with benzopirene and also treated with the PGL. Next, an examination was conducted by using immunohistochemical techniques. Results: The results then showed that the provision of the PGL could decrease the expression of Bcl-2 significantly higher than that of EA in the malignant epithelial cells of the oral mucosa of those mice. Conclusion: It may be concluded that the provision of the PGL can kill malignant cells in the oral cavity of mice by increasing apoptosis through decreasing Bcl-2 expression that was higher than the provision of EA.
Keyword :
Pomegranate fruit extract, malignant cells, Bcl-2, ellagic acid ,
References :
Epstein W,(2008) Oral cancer. Texbook of oral medicine - : BC Decker Inc Hamilton Press
Archive Article
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 Volume : 46 / No. : 1 / Pub. : 2013-03 |
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