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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 43 / No. 3 / Published : 2010-09

TOC : 9, and page :146 - 150

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Original Article :

The molecular pheomena of  the blaz genes   forming beta-lactamase enzymes structure in staphylococcus aureus resistant to beta-lactam antibiotics (ampicillin)

Author :

  1. Mieke Satari *1
  1. Department of Oral Biology Faculty of Dentistry, Padjadjaran University Bandung - Indonesia

Abstract :

Background: Nowadays, infectious diseases still becomes problems. One of the bacteria causing infectious diseases is S. aureus. In the effort to deal with infections caused by S. aureus, beta-lactam antibiotics, such as ampicillin, are used. In fact, it is unfortunately known that many of S. aureus bacteria are resistant to this group of antibiotics. One of the reasons is known because of nucleotide base changes in the structure of the genes (blaZ) which encode beta-lactamase enzymes in S. aureus. Purpose: The objective of this study is to analyze the nucleotide base changes in the structure of the genes (blaZ) forming beta-lactamase enzymes in S. aureus resistant to ampicillin based on molecular point of view. Methods: Molecular examinations was conducted by isolating the genes, forming beta-lactamase enzyme, which length was 845bp, from 7 isolates of S. aureus resistant to ampicillin by using PCR technique. The results of blaZ amplification were then subjected to homology by using  Tn 552 of S. aureus  obtained from Bank of Genes. Rresults: Based on the result of the homology, it was found that there was a change in purine base TG, which was a pyrimidine base at the -37 position of the initial codon of blaZ. This change, however, did not affect the strength of the promoter since the number of A and T is still more than the number of G and C. In the  structure of the gene (blaZ) there was even no mutation or deletion or nucleotide base substitution found, so it would not affect the effectiveness of beta-lactamase enzyme. Conclusion: It can be concluded that the resistance of  S. aureus  towards ampicillin is not caused by nucleotide base deletion/substation. Instead, it is suspected that there are other causes leading to the resistance, including the overproduction of beta-lactamase enzyme, causing the degradation of beta-lactam antibiotics conducted by the beta-lactamase enzyme.

Keyword :

S. aureus, ampicillin, blaZ ,


References :

  1. Peterson LJ, (1998). Principles of management and prevention of odontogenic infection . St Louis : Mosby Year Book Inc


   


Archive Article

Cover Media Content

Volume : 43 / No. : 3 / Pub. : 2010-09
  1. Computer aided diagnosis for osteoporosis based on trabecular bone analysis using panoramic radiographs
  2. Anti-inflammation effects of sardinella longicep oils against paw oedema on rattus novergicus induced by 1% carrageenan
  3. The correlation between immunoexpression of estrogen receptor and the severity of periodontal disease
  4. The effectiveness of sharp end and rounded end bristle toothbrush
  5. Contact hypersensitivity after using  tongue piercing
  6. Cervical end preparation design of collarless metal ceramic crown to the decrease of bacterium colony number
  7. Treatment of sharp mandibular processus alveolaris with hybrid prosthesis
  8. The combination of miacalcic, calcium lactate, and vitamin c as post-extraction alveolar bone resorption inhibitor
  9. The molecular pheomena of  the blaz genes   forming beta-lactamase enzymes structure in staphylococcus aureus resistant to beta-lactam antibiotics (ampicillin)
  10. Management of idiopathic alveolar bone necrosis associated with oroantral fistula after upper left  first molar extraction
  11. Indirect veneer treatment of anterior maxillary teeth with enamel hypoplasia