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Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 42 / No. 1 / Published : 2009-01

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Original Article :

Toxicity testing of chitosan from tiger prawn shell waste on cell culture

Author :

  1. Maretaningtias Dwi Ariani*1
  2. Anita Yuliati*2
  3. Tokok Adiarto*3
  1. Department of Prosthodontic, Faculty of Dentistry Airlangga University
  2. Department of Dental Material, Faculty of Dentistry Airlangga University
  3. Department of Chemistry, Faculty of Sience & Technology Airlangga University Surabaya – Indonesia

Abstract :

Background: A biomaterial used in oral cavity should not become toxic, irritant, carcinogenic, and allergenic response. Chitosan represents a new biomaterial in dentistry. Purpose: To examine the toxicity of chitosan from tiger prawn shell waste on cell culture with MTT assay. Methods: Chitosan with concentration of 0.25%, 0.5%, 0.75% and 1% was used in this experiment. Each of sample was immersed on eppendorf microtubes containing media culture. After 24 hours, the immersion of media culture was used to examine the toxicity effects on BHK-21 cell based on MTT assay method. The density of optic formazan indicates the number of living cells. All data were then statistically analyzed by one-way Anava. Results: The number of living cells in chitosan from tiger prawn shell waste was 93.16%; 85.07%; 78.48%; 75.66%. Thus, there was no significant difference among groups. Conclusion: Chitosan with 0.25%, 0.5%, 0.75% and 1% concentrations from tiger prawn shell waste were not toxic for BHK-21 cell culture when using parameter CD50.

Keyword :

Toxicity, chitosan, MTT assay,

References :


    Archive Article

    Cover Media Content

    Volume : 42 / No. : 1 / Pub. : 2009-03
    1. The Transformation Of Ordinal Scale For Parametric Statistic Analysis On Dental Health Questionnaire
    2. Chronic Gingivitis And Aphthous Stomatitis Relationship Hypothesis: A Neuroimmunobiological Approach
    3. The Role Of Microendodontics In Treating Mandibular Second Molar With Five Canals
    4. Toxicity Testing Of Chitosan From Tiger Prawn Shell Waste On Cell Culture
    5. The Effect Of Toothpaste Containing Kayu Sugi Extract On Plaque Formation
    6. Simple Replantation Protocol To Avoid Ankylosis In Teeth Intended For Orthodontic Treatment
    7. Treatment Of Temporomandibular Dissorder’s Using Occlusal Splint
    8. Inhibitory Effect Of N-hexane : Ethyl Acetate Fraction From Artemisia Vulgaris L. On Cell Culture Of Epithelial Carcinoma Of The Mouth
    9. Antioxidant Effect Of Minocycline In Gingival Epithelium Induced Toxin Actinobacillus Actinomycetemcomitans Serotype B
    10. Esthetic Rehabilitation Of Crowded And Protruded Anterior Dentition
    11. Patient Safety Oriented To Improve Patient Retention In Oral Health Services