UNIVERSITAS AIRLANGGA



Detail Article

Dental Journal (Majalah Kedokteran Gigi)

ISSN 1978-3728

Vol. 42 / No. 2 / Published : 2009-04

Related with : Scholar   Yahoo!   Bing

Original Article :

Transformation analysis of oral epithelial dysplasia to cis and scc by p53 expression and gene mutations

Author :

  1. Mei Syafriadi*1
  1. Divisi of Pathology, Department of Biomedical Sciences
    Faculty of Dentistry, Jember University
    Jember - Indonesia

Abstract :

Background: It is known that oral squamous epithelial dysplasia (SED) and carcinoma in-situ (CIS) are precancerous lesion and it could transform to squamous cell carcinoma (SCC). We had been reported p53-Protein Over-Expression and Gene Mutational of Oral CIS, such as basaloid, verrucous, and acanthothic/atrophic types, but demarcated between SED to CIS and CIS to SCC and how their transformation is still unclear. We considered that their molecular behavior related each other. Purpose: To understand the molecular behavior of them we examined p53 exon 5-8 gene mutation and their protein expression in the sequential cases. Methods: Using 10 cases formalin–fixed paraffin sections that composed SED appearance, CIS and SCC in the same case were subjected to P53 immunohistochemistry. Then all cases were subjected to p53 gene mutations analysis. By laser capturing microdissection dysplasia part, CIS part and SCC part were cutted, and followed by direct sequencing of PCR product for exon 5-8. Result: SED p53-protein over-expression in some cells, and the expression was increased to CIS and SCC. Mutational analysis for p53 gene showed that 60% of p53 gene mutation in CIS also found in SCC, therefore SCC had additional mutation in other exon of p53 gene. While no particular mutations were found in SED part of all cases. Conclusion: Carcinoma in-situ is a squamous cell carcinoma eventhough not invasive yet, but squamous epithelial dysplasia is an early step to malignancy. It needs other genes examination to know any genes are involved in the precancerous to cancer transformation process.

Keyword :

dysplasia, CIS, SCC, mutation, p53 ,


References :





Archive Article

Cover Media Content

Volume : 42 / No. : 2 / Pub. : 2009-06
  1. Diagnosis And Management Of Crohn’s Disease In Retarded Child
  2. Pseudomembranous Candidiasis In Patient Wearing Full Denture
  3. Cooperation Of Patient As Key Factor To Overcome Oral Habits
  4. Transformation Analysis Of Oral Epithelial Dysplasia To Cis And Scc By P53 Expression And Gene Mutations
  5. Aesthethic And Masticatory Rehabilitation On Post Mandibular Resection With Combination Of Hollow Obturator And Hybrid Prosthesis
  6. The Effect Of Watermelon Frost On Prostaglandin E2 (pge2) In Inflamed Pulp Tissue (in Vitro Study)
  7. Shear Bond Strength Between Porcelain And Nano Filler Composite Resin With Or Without 9% Hydrofluoric Acid Etching
  8. Screening Of Oral Premalignant Lesions In Smokers Using Toluidine Blue
  9. Biocompatibility Of Acrylic Resin On Bhk–21 Cell Line After Being Soaked In Sodium Hypochlorite
  10. The Aesthetic Treatment For Anterior Teeth With Lost Crown, By Endorestoration
  11. Prognostic Value Of Molecular Markers Of Oral Pre-malignant And Malignant Lesions

Information DENTJ