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Folia Medica Indonesiana

ISSN 0303-7932

Vol. 46 / No. 4 / Published : 2010-10

TOC : 4, and page :253 - 256

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Original Article :

2-methoxyethanol exposure affects protein profile of testicular sperm membrane in mice (mus musculus)

Author :

  1. Alfiah Hayati*1
  2. Amilia Mahmuda*2
  3. IB Rai Pidada*3
  1. Department of Biology, Faculty of Science and Technology, Airlangga University
  2. Department of Biology, Faculty of Science and Technology, Airlangga University
  3. Department of Biology, Faculty of Science and Technology, Airlangga University

Abstract :

The 2-Methoxyethanol (2-ME) is a one of the toxic agents which affect on male reproduction from phthalate ester. The 2-ME is used for making resins, lacquers and paint in industrial solvent and may enter in the body through skin, by inhalation or per oral. The aim of this study was to determine the effect of 2-ME in varied doses toward sperm membrane protein of mice testis. This study used male BALB/C strain mice (Mus musculus), aged 4-5 weeks with 24-30 g weight. Based on the types of treatment, the subjects were divided into 3 groups. One control group was injected with physiological saline solution and 2 treatment groups were injected with 2-ME dose respectively 50 and 100 mg/kg. All groups were injected daily for 12 days by subcutaneous. Spermatozoa membrane protein was taken from testicular spermatozoa. The protein profiles of spermatozoa membrane were analyzed by sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE 15%) techniques. Results of this research showed the existence of differences between profile of spermatozoa membrane protein in control group and treatment group. In control and treatment group receiving a dose of 50 mg/kg body weight there was around 15 protein bands with variation of the molecular weight between 93.3-7.4 kDa. Whereas, in treatment group receiving dose of 100 mg/kg body weight there was 14 protein bands with 60 kDa protein not expression. Conclusion of this research was that the exposure of 2-ME can affect the protein profile.

Keyword :

2-Methoxyethanol, protein profile, testicular spermatozoa,


References :

  1. Barrios B, Perez-Pe R, Gallego M, Tato A, Osada J, Blanco T, Perez JAC, (2000). Seminal plasma protein revert the cold-shock damage on ram sperm membrane . - : Biology of Reproduction
  2. Chitra KC, Sudjatha R, Latchoumycandane C, Marthur PP, (2001). Effect of lindane on antioxidant enzymes in epididymis and epididymal sperm of adult rats. - : Asian Journal of Andrology
  3. Dukan S, Farewell A, Ballesteros M, Taddei F, Radman M, Nystrom T, (2000). Protein Oxidation in Response to Increased Transcriptional or Translasional Errors. Cambridge : Massachusetts Institute of Technology


   


Archive Article

Cover Media Content

Volume : 46 / No. : 4 / Pub. : 2010-10
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  3. Combination of metformin and hyperbaric oxygen therapy increased enos concentration
  4. Description of blood viscosity from diabetes mellitus outpatients in endocrine clinic, aw sjahranie hospital, 2009
  5. 2-methoxyethanol exposure affects protein profile of testicular sperm membrane in mice (mus musculus)
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